493 
1907-8.] On the Effects on Metabolism of Chloroform. 
administration of chloroform by the respiratory passages had shown so 
close a correspondence between the results of the methods of Bohland 
and of Morner and Sjoqvist, the former method was abandoned in the 
present series of analyses, for a divergence between these would have more 
definitely proved that the undetermined nitrogen is really in amino acids. 
That the rise in the proportion of undetermined nitrogen was not due to 
the appearance of protein is indicated, first, by the fact that in Experiment 
VII. the rise occurred before the appearance of the protein, and, second, by 
CO(N'H 2 ) 2 '| 
nh 3 Uv 
Residual J 
Fig. 1 . — Experiment VII. 
Fig. 2. — Experiment VIII. 
the fact that the amount of protein was insufficient to account for the 
nitrogen thus excreted. 
The proportion of unoxidised sulphur to the total sulphur (Experiment 
VII.) shows, like the nitrogen in urea, a distinct fall, curiously interrupted 
by a rise on the two days on which the proportion of urea nitrogen was 
lowest. I am unable to explain this. 
The whole series of changes is such as would be produced by a direct 
toxic action on the liver, similar to that produced by the administration 
of alcohol and sulphonal (Jour, of Phys., xxvi., p. 166, 1901). The effect is 
not immediate, but is nevertheless the result of the poisoning of the cells. 
This is clearly shown by the histological changes produced in the liver 
cells by giving chloroform by the mouth, already described by Doyon and 
