498 
Proceedings of the Royal Society of Edinburgh. [Sess. 
The following table gives results obtained when working with known 
quantities of chloroform : — 
Amount CHC1 3 taken. 
Amount CHC1 3 found. 
Percentage. 
11 '9 mgrs. 
11 *8 mgrs. 
99-1 
12 „ 
11*9 „ 
99-1 
12 „ 
11*85 „ 
98-75 
49-8 „ 
49 „ 
98-3 
Two or three times during the course of the investigation blood was 
taken from animals to which no chloroform had been given, and in each 
of these cases negative results were obtained. 
Chloroform by the Respiratory Passages. 
The taking up and elimination of chloroform from the blood when 
given by the respiratory passages has been investigated by several 
observers. 
Grehant and Quinquand ( G . R., 1883, p. 753) found that to produce anaes- 
thesia in dogs the chloroform in the blood must amount to something like 50 
mgrs. per 100 c.cm. These observations were confirmed by Nicloux (C. R. 
Soc. biol., T. lx. p. 144), who further showed that after the administration 
was stopped the elimination went on, at first rapidly and then more slowly, 
and that even after three hours 7*5 mgrs. might be present. 
Buckmaster and Gardiner ( Proc . Roy. Soc., vol. lxxix., 1907), working 
upon cats, found that the amount of chloroform was about 20 or 30 
mgrs. per 100 c.c.’s when anaesthesia, as indicated by the corneal reflex, 
was produced. They also showed that the chloroform is first rapidly 
and then more slowly taken up by the blood until a state of equilibrium 
between assumption and elimination is established, so that continued 
administration does not necessarily further increase the percentage of 
chloroform in the blood. 
Brodie and Widdows {Brit. Med. Journ., vol. xi., 1906, p. 79) have 
recorded somewhat similar results. 
Tissot (C. R. Soc. biol., T. lx., 1906, p. 198) shows that the proportion of 
chloroform in the blood at the onset of anaesthesia depends upon the rate 
at which it is given. If the animal is anaesthetised, in three or four 
minutes the amount may be as much as 60 or 70 mgr. per 100 c.c., but if 
the chloroform is given drop by drop and the anaesthesia slowly produced, 
the amount may be only 34 or 35 mgr. or even less per 100 c.cm. As he 
