1889 - 90 .] Dr Griffiths on Researches on Micro-Organisms. 261 
From these details, it appears that the medium in which the un- 
discovered microbe of rabies resides is essentially nervous matter, 
and not in the blood system. Hence the hypodermic injection 
method is useless as a means for preventing or curing this terrible 
disease. 
(d) Various Micro-Organisms. 
The following micro-organisms — 
(1) Bacillus fioccus (obtained from garden soil), 
(2) Bacillus toruliformis (obtained from garden soil), 
(3) Bacillus tardecrescens (from ammonium carbonate solution), 
(4) Micrococcus candicans (from the atmosphere), 
(5) Micrococcus rosaceus (from the atmosphere), 
(6) Micrococcus chlorinus , — - 
as well as those mentioned in my last paper ( loc . cit.) on this 
subject, have all been destroyed by the germicides already men- 
tioned. 
III. Vitality of Certain Micro-organisms. 
In continuation of the experiments recorded in Part II. of this 
paper, I have found that the vitality of certain micro-organisms is 
considerable. 
Portions of a pure cultivation of Micrococcus chlorinus (growing 
on white of egg) were mixed with calcium sulphate and calcium 
carbonate (the mineral ingredients were previously sterilised at a 
temperature of 135° C.) and placed in a number of sterilised tubes, 
which were then hermetically sealed. Twelve of these tubes each con- 
tained from 8 to 10 grammes of the mixture. Twelve sterilised tubes, 
not hermetically sealed (see Part II. of this paper), also contained the 
same quantity of the mixture. The twenty-four tubes were kept at 
a dry heat of 32° C. from one to eight months. Two hermetically 
sealed tubes and two of the “ open ” tubes were opened after an 
exposure at 32° C. for one month. Four tubes containing sterilised 
nutrient beef-broth were inoculated from the contents of the tubes. 
In the two inoculated from the “open” tubes, growths of Micro- 
coccus chlorinus (proved by staining, microscopical and macroscopical 
appearances) made their appearance after nine days’ incubation. 
Growths of Micrococcus chlorinus also made their appearance in the 
