366 
Proceedings of Royal Society of Edinburgh. [sess. 
Expi. 27. — Frog, 27 grms. B., 21 in 10 seconds. 
12.10. — 001 grm. methocodeine hydrochloride into abdominal 
cavity. 
12.20. — Has become much less active. 
12.30. — B., 14; very shallow. Only moves if pinched. 
12.50. — B. ceased. Lies on back if turned over. Draws up leg 
if severely pinched. 
1.20 — Faint response if severely pinched. Sciatic nerves excit- 
able to very weak current. 
3.20. — Frog’s muscles all over body are much depressed in irri- 
tability. Many of them do not contract when stimulated with 
strongest electric current, others only very feebly. Motor nerves 
still excitable, for, when stimulated, the muscles which are not in 
complete rigor respond faintly. Heart stopped. Corneal reflex 
still present. 
In many cases the poisoning of the muscles took place more 
slowly, death occurring only after some days, hut in all the general 
symptoms were the same. 
One centigramme proved fatal to frogs sooner or later. With 
larger doses also the results are the same. 
Expt. 28. — Frog, 28 grms. B. in 10 seconds, 23. 
12.10. — 0 - 025 grm. under skin of abdomen. 
12.25. — Has become much depressed; moves away heavily if 
pinched. 
1.0. — Lies in any position; still responds to severe pinching. 
1.40. — Sciatic nerves excitable to very weak currents (250 mm.). 
2.45. — Sciatics same. Muscles of abdominal wall and left thigh 
are in rigor mortis ; other muscles contract on electric stimulation. 
5.30. — Muscles and sciatic nerves much diminished in electric 
irritability. 
Next day. — Heart stopped in marked systole ; none of the volun- 
tary muscles contract to electric stimulation. 
An increase of reflexes was never observed. Direct application 
of a solution to the exposed spinal cord or injection into the aorta 
depressed the reflexes or entirely abolished the vitality of the cord 
in a very short time. 
In frogs small doses of 5 milligrammes depress the power and 
rate of the heart. 
