SIGNIFICANCE OF COLON COUNT IN MILK. 
Table 5. — Colon count from milk produced under clean conditions. 
11 
Sample No. 
Total 
count. 
Colon count. 
Fresh. 
After 24 hours 
at 70° F. 
(21.1° C). 
1 
Per c. c. 
6,200 
6,200 
8,900 
5,100 
3,900 
5,400 
3,600 
2,200 
6,500 
4,600 
6,300 
3,300 
4,700 
1,130 
2,270 
5,300 
Per 1J10 c. c. 
















Per c. c. 
12,000 
5,000 
16,000 
9,000 
125,000 
51,000 
24,000 
900 
3,300 
88,000 
6,000 
54,000 
35,000 
303,000 
291,000 
83,000 
2 
3 
4 
5 
6 
7 
8 
9 
10 
11 
12 
13 
14 
15 
16 
Further examination was made of 14 samples of certified milk as 
shown in Table 6. According to this table only 7 of the 14 samples 
showed the presence of colon organisms in 1 cubic centimeter of 
fresh milk, as determined in lactose-broth tubes; but after 24 hours 
they were found in every sample. These results, together with those 
of other investigators, clearly indicate that milk as commercially 
produced under the most careful conditions is never entirely free 
from organisms of the colon- aerogenes group. 
Table 6. — Colon count from certified milk. 
Sample No. 
Total 
count in 
milk as 
delivered. 
Colon count. 
Fresh. 
Held 24 hours at— 
Held 48 hours at— 
60° F. 
(15.6°C). 
70° F. 
(21.1° C). 
60° F. 
(15.6° C). 
70° F. 
(21.1° C). 
1 
Per c. c. 
7,400 
10,400 
5,200 
10, 100 
8,200 
4,200 
12, 600 
5,900 
14, 800 
11,400 
10, 100 
22, 800 
7,800 
15, 700 
Per c. c. 
10 
1 1 
i 1 
20 

i 1 
10 
11 






Per c. c. 
10 
110 
10 
280 
100 
170 
320 
20 


460 
10 
20 
150 
Per c. c. 
4,600 
38,000 
2,900 
79, 000 
400 
16,600 
13,500 
1,800 
3,100 
400 
38,000 
1,100 
6,500 
5, 800 
Per c. c. 
23,700 
49,000 
46-000 
55,000 
1 900 
120,000 
169,000 
21,600 
2,700 
4,600 
186.000 
6,400 
10. 800 
36,000 
Per c. c. 
4,400,000 
15,000.000 
2,890,000 
16,400,000 
1, 130, 000 
6, 100. 000 
12,500,000 
560, 000 
610,000 
5,300,000 
13,100,000 
3,700.000 
8,700,000 
1,510,000 
2 
3 
4 
5 
6 
7 
8 
9 
10. 
11.. 
12 
13 
14 
Average. 
10,471 
119 
15, 121 
52,336 
6,564,286 
1 means at least 1 per cubic centimeter. 
