4 BULLETIN 1299, U. S. DEPARTMENT OF AGRICULTURE 
selections of hard red winter wheat also was inoculated, and the plants 
were grown in the nurseries at Moro in 1919. 
In 1920 experiments were conducted in California as well as in 
Oregon and Washington, and were continued in California in 1921. 
Artificially smutted seed of the above-mentioned varieties and selec- 
tions, together with that of additional ones, was sown at Moro, Oreg., 
Pullman, Wash., and Davis, Calif. Many of the Australian and 
Indian wheats also were grown in this season. At Davis about 950 
varieties and selections were sown. The experiments at Moro were 
somewhat less extensive, and still fewer varieties and selections were 
sown at Pullman. 
These experiments were continued for two years at each of the three 
stations, and as nearly as possible the same varieties were included in 
the sowings at all points. Many of the data obtained at Moro have 
been published by Stephens and Woolman (9) in a bulletin issued by 
the Oregon Agricultural Experiment Station. Sowings of a limited 
number of these wheats were made at Corvallis, Hermiston, and 
Union, Oreg., for the purpose of determining the effects of local 
conditions on bunt infection. These latter data are not given in the 
tables because the results obtained did not permit the drawing of 
accurate conclusions. 
METHODS 
The seed of these numerous varieties and pure-line selections to be 
sown at the different stations was obtained as far as possible from 
identical seed lots and then heavily smutted with spores oiTiUetia 
tritici before sowing. The seed, together with dry spores produced 
in the previous year, was thoroughly shaken in a closed container 
until the surface of each kernel had a dark smutty appearance. The 
seed was sown then in single, duplicated, or triplicated rows about 1 
foot apart. The sowings were made at each station as nearly as 
possible at the time when conditions would be conducive to maximum 
infection. This time, of course, varied considerably, depending on 
the locality and existing weather conditions. At Davis there was 
considerably more bunt in these wheats in the crop of 1920 than in 
that of 1921. This no doubt was caused by the difference in weather 
conditions at the time of sowing. 
In determining the percentages of bunt, counts of the smutted heads 
and of total heads were made and the percentage of bunt calculated 
from these figures. In most cases both plant and head counts were 
made, but the percentages given in the tables in this bulletin are 
based on head counts only, as these figures seem to indicate more 
accurately the actual damage caused by the disease. 
A list of the varieties and selections grown at Davis, Moro, and 
Pullman, showing the percentages of bunt infection, is given in Table 
1. The list does not include the Australian, Indian, and South 
African wheats, which are given in Tables 4, 5, and 6, or a number of 
selections from White Australian. 
Most of these wheats were grown during two years. Lack of funds 
prevented the continuation ol these experiments for a longer period. 
However, results for two years at these widely separated stations 
should be sufficient to furnish a fairly satisfactory indication of the 
relative resistance of these wheats to bunt. When a variety or seiec- 
