84 BULLETIN 754, U. S. DEPARTMENT OF AGRICULTURE. 
The colored horny combination in the male gametes was in excess _ 
by 17 and deficient in the female gametes by 21; so a deficiency of 4 | 
would be expected on the self-pollinated ear, and we find a defi- 
ciency of 8. ; 
The colored waxy combination in the male gametes is deficient by | 
18; in the female gametes this same combination is in excess by 8; | 
so a deficiency of 10 would be expected in the self-pollinated ear, | 
and a deficiency of 18 is noted. | 
The white horny combination is deficient by 17 in the male | 
gametes and in excess by 17 in the female gametes; so that they _ 
would be expected in the theoretical proportion on the self-pollinated | 
ear, and we find an excess of 6. This agreement of evidence derived | 
from so many different sources would indicate that the departures | 
observed were the result of a definite and unexpected gametic series | 
formed by the plant which bore ears Nos. 1799 and 1800, rather than | 
fluctuations due to chance. | 
The three ears that were obtained when plants grown from white | 
waxy seeds were used as the male parent had a total of 1,293 seeds | 
with a correlation of 0.610+0.025, but since one ear of the three | 
has been shown to be of entirely different coupling this ear should | 
not be included in the totals. Omitting, then, this one ear, No. 
1795, which is outstanding and does not belong to the series, the | 
total number of seeds then becomes 740, with a correlation of | 
0.737+0.025—a deviation from the expected 0.8 of but 2.5 times the | 
probable error. | 
Nine ears were secured from plants grown from the colored waxy | 
seeds as the male parent, and two ears were secured from plants — 
grown from the white horny seeds as the male. These ears are also 
shown in Table XXXVI. The correlation for the total number of | 
seeds is not significant. One ear, however, No. 1781, has a negative | 
association between C and H of 0.220+0.059, a deviation from a 
zero correlation by 8.7 times the probable error. This ear could in all - 
probability be looked upon as the result of chance fluctuation in — 
sampling, though closely approximating the 0.27 expected when a> 
plant heterozygous for the color factors C and Ff and horny 
endosperm texture with a 3-1-1-8 coupling between the factor C and 
waxy endosperm is crossed with a homozygous waxy plant heter- | 
ozygous for the two factors for color. | 
As a test of the possibilities of securing a correlation when cross- | 
ing two plants, one heterozygous for endosperm texture and without | 
the factor for color C, the other homozygous for endosperm texture | 
and heterozygous for the factor C’, crosses were made between plants — 
from white horny seeds and plants from colored waxy seeds. These — 
crosses were made, using the colored waxy plants as the female parent | 
and also as the male parent. | 
