10 BULLETIN 754, U. S. DEPARTMENT OF AGRICULTURE. 
Since the deviation observed was approximately 1 per cent, only 
one ear of every hundred would be expected to be all waxy. Several 
hundred ears, the result of self-pollinating plants grown from the 
horny seeds of waxy X horny hybrids, have been secured, but no 
all-waxy ears have been found. 
The shortage of waxy seeds not only occurs where the expected 
ratio of horny to waxy is 3 to 1, but was also observed in crosses 
between waxy endosperm and aie: endosperm where the Mendelian 
dihybrid ratio was expected (8). Here the deviation was 1 per cent 
below the expected 18.75, and it should not occur as the result of 
chance oftener than once in 15,000 times. 
THIRD GENERATION OF THE HYBRID DH 234. 
Three ears were selected for planting from the hybrid Dh 284. 
These three ears are Nos. 1099, 1110, and 1111 in Table I. The 
last two ears were borne on the same plant. Ear No. 1110 repre- 
sents a cross between the two hybrids and is discussed later, with 
the results from a similar ear taken from the hybrid Dh 2387, 
One of the two ears remaining, No. 1111, was self-pollinated, while 
No. 1099 was the result of pollen from another plant of the same 
hybrid. Both of these ears were close approximations of the expected 
25 per cent waxy, as was also a self-pollinated ear secured from the 
male parent of No. 1099. 
There were four classes of seeds on Nos. 1099 and 1111, colored and 
white horny and colored and white waxy. These classes were planted 
separately, and reciprocal crosses were made between white waxy 
and colored horny and colored waxy and white horny plants. Wher- 
ever possible, self-pollinated ears were secured from all the classes, 
but since the self-pollinated plants grown from waxy seeds resulted 
in ears all the seeds of which were waxy, these ears do not appear in 
the tables, | 
To avoid unnecessary complications, the inheritance of aleurone 
color is discussed separately from the behavior of the texture of 
the endosperm. The color of the aleurone appeared not to affect the 
behavior of the waxy endosperm as far as the percentage of waxy 
seeds was concerned. The progenies were examined with this point 
in mind, but since no differences were found it was not thought nec- 
essary to discuss the behavior of the colored seeds separately from 
the behavior of the white seeds. 
The results obtained from the progeny of ears Nos. 1099 and 1111 
were also examined separately, but presented no significant difference 
in their behavior, and to avoid unnecessary repetition the progeny 
of these two ears will be considered together. The progenies of the 
four classes of seeds from the two ears are separately indicated in 
Tables III and IV. 
