8 BULLETIN 1262, U. S. DEPARTMENT OF AGRICULTURE. 
size being infected with Polyporus schweinitzi, Trametes pini, and 
Fomes laricis, respectively. 
Upon removal from the kiln most of the pieces showed a consider- 
able area covered with mold, which had developed during the test. 
The test pieces of Douglas fir of the 6 by 6 by 24 and the 8 by 8 by 
24 inch sizes, freshly cut, showed green-mold growth covering the 
sapwood. It is apparent that the conditions were favorable to mold 
growth. 
At the end of the test the cultural methods showed the fungi in 
most of the test pieces to be dead. Positive cultures developed as 
follows: 
For the 4-inch pieces—blue stain in Pinus strobus and Thuja occidentalis, 
Ganoderma tsugae in Tsuga canadensis, Trametes pini in Larix laricina, 
Pholiota adiposa in Tilia americana, Polyporus borealis in Acer saccharum, 
Polyporus amarus in Libocedrus decurrens, Polystictus hirsutus and P. versi- 
color in Alnus oregond. 
For the 6-inch and 8-inch pieces—Fomes laricis and Polyporus schweinitzii 
in Pseudotsuga tazvifolia, 
RUN 7. 
Five sets of test pieces of the Neopit material were stacked in a 
Tiemann dry kiln. Steam was turned on gradually into the kiln 
until the recording instruments showed a temperature of 120° F. 
and a relative humidity of 100 per cent. The test was begun at this 
point, and this temperature and humidity were maintained. 
A set of the test pieces was removed at the end of 3, 6, 9, 12, and 24 
hours. Culture blocks were cut from the centers of each of the test 
pieces, and fragments from each block were cultured to determine the 
viability of the fungi. : 
The set of test pieces subjected to 120° F. at 100 per cent humidity 
for three hours upon culturing showed most of the cultures positive. 
In the 1-inch pieces the following were positive: Blue stain in 
Pinus strobus, brown ring-rot in Z’huja occidentalis, Torula ligni- 
perda in T’suga canadensis, Fomes pinicola in Tsuga canadensis, 
Fomes fomentarius in Betula papyrifera, brown cubical ®t in 7 suga 
canadensis, Trametes pint in Pinus strobus, and Polyporus borealis 
in Acer saccharum. The following were negative: Blue stain in 
Thuja occidentalis, Trametes pint in Larix laricina, brown cubical 
rot in Thuja occidentalis, Ganoderma tsugae in Tsuga canadensis, 
Fomes ignaarius in Ulmus americana, brown cubical rot in Pinus 
strobus, Fomes igniarius in Acer rubrum, and Pholiota adiposa in 
Tilia americana. 
The blue-stain organisms remained alive in all three sizes of test 
pieces, 1-inch, 2-inch, and 4-inch, during the 3-hour test in the wood 
of Pinus strobus. The blue-stain organism in the 1-inch and 2-inch 
test piece of 7huja occidentalis when cultured did not revive. The 
heartwood of this particular test piece was infected with a wood 
destroyer producing a brown crumbly or cubical rot. 
In general, all the 4-inch test pieces gave positive cultures. Of 
the 2-inch pieces 13 gave positive and 3 negative results. The re- 
sults indicate that the time and temperature factors used with this 
set are not practicable in killing wood-inhabiting fungi in 1-inch, 
92-inch, and 4-inch stock. 
The set removed from the kiln at the end of six hours upon cultur- 
ing showed, in general, that the fungi were killed in all of the 1-inch 
