EFFECT OF KILN DRYING, ETC., ON FUNGI IN WOOL. 7 
The fungi were found to pe dead within the centers of all the 1- 
inch, 2-inch, and 4-inch pieces. 
RUN 4. 
A set of test pieces of the Neopit material was placed in a Tie- 
mann dry kiln for a period of 18 days. The set was placed on top of 
a charge of 1-inch commercial birch lumber. The temperature range 
during the run was from 135°, the initial temperature, to 180° F. 
The range in relative humidity was from 50 to 85 per cent. On the 
seventeenth day of the run the charge was conditioned for 20 hours 
at 180° F. and 85 per cent relative humidity. 
At the end of the run, cultures made from these blocks showed all 
the fungi to be dead within the center of each test piece. 
RUN 5. 
(a) Six green 1-inch sap gum (Liguidambar styraciflua) boards 
showing blue stain were subjected to 380 pounds of steam, gauge pres- 
sure at approximately 274° F., for a period of 40 minutes in a 
Kraetzer preparator. Samples containing blue stain were cut from 
these boards after the treatment and forwarded from Memphis, 
Tenn. 
Cultures made by using fragments of the blued wood taken from 
the center of each board showed the fungus to be dead in each case. 
(b) Six blocks of pitch pine (Pinus rigida), each measuring 1 by 
2 by 2 inches and infected with the brown-stain fungus (A/lternaria 
sp.) were subjected to a so-called dry heat of 221° F. for a period 
of 24 hours. Cultures showed the fungus to be dead in all of the 
blocks at the end of the test. | 
(c) Disks cut transversely from the central part of two jack-pine 
(Pinus bankstana) railroad ties which had been treated with creosote 
and subjected during the treatment to a temperature of 178° to 188° 
F. for a period of 47 to 50 minutes were sent to the writer for exami- 
nation. 'TWe ties were infected before treatment and showed the 
typical stage of rot characteristic of 7’rametes pint. Disks cut from 
several untreated ties and showing various stages of the same rot 
were also received. Cultures made from the central areas in the 
various disks showed the fungus to be alive in the central part of the 
treated disks as well as in the untreated ones. The treated ties were 
7 by 84 inches and 8 by 94 inches in cross section, respectively. 
(d) Two sets of test pieces from the Oakridge material were sub- 
jected to 20 pounds of steam (gauge pressure, 259° F.) followed 
by a vacuum of 1 hour. The first set was steamed for 14 hours and 
the second set for 2 hours. The results show that the 14-hour treat- 
ment killed the organisms in the 6-inch but not in the 8-inch pieces. 
The 2-hour treatment killed the organisms in both sizes. 
RUN 6. 
A set of test pieces from the Neopit and Oakridge material was 
placed on stickers in an open pile in a Tiemann dry kiln. The 
pieces were subjected to a temperature of 110° to 116° F. at saturated 
atmosphere for a period of 48 hours. This set contained three 6- 
inch and three 8-inch test pieces of Douglas fir, the pieces of each 
