20 BULLETIN 306, U. S. DEPARTMENT OF AGRICULTURE. 
the progeny through seed and whether the character is changed by 
vegetative propagation. The results thus far show that the first- 
generation plants secured from seed of cross-pollinated selected — 
individuals display the characteristic of the maternal parent with — 
regard to alkaloid productivity. This condition is generally true 
at all stages of growth during a season and also for at least two suc- 
cessive seasons. Close pollination of the parent plant has shown 
only a moderate influence on the transmission of this characteristic. — 
Second-generation plants from cross-pollination have been grown — 
at Arlington, Va., Madison, Wis., and Timmonsville, S. C., and at all 
three stations they have displayed the relative alkaloid-producing 
tendencies evident in the original parent plant and the generation 
preceding. | 
While the plants at the different localities showed a parallel rela- 
tionship toward each other, there was considerable difference in the 
general quantity of alkaloids produced. Thus, in the case of Madison ~ 
and Arlington, where two pickings were made at fairly corresponding 
stages of growth, it was found that the Madison plants yielded more 
alkaloids than those at Arlington. At Timmonsville the yield was 
still greater than at Madison, but here only one picking was made, 
and it is hardly possible to make a true comparison. Nothing definite — 
developed to indicate that a relationship exists between the amount 
of precipitation and sunshine and the percentage of alkaloids pro- 
duced. | 
Plants were grown from cuttings, and at two stages of their growth 
these plants showed a marked tendency to display the same charac- 
teristic regarding alkaloid production as the plants from which they _ 
were propagated and the original parents of those plants. 
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