18 
BULLETIN 306, U. S. DEPARTMENT OF AGRICULTURE. 
REPRODUCTION OF SELECTED PLANTS FROM CUTTINGS. 
In the fall of 1913 cuttings were secured from the first-generation 
plants and rooted in the greenhouse. Although the season had been 
bad and the condition of the cuttings was rather poor, a good per- 
centage of them rooted and by spring furnished plants 3 to 4 inches 
high. These were transplanted to the field early in May. The severe 
drought during that month caused a considerable number of the 
plants to die, although they had developed a good root system. On 
August 18, when the flowering was mostly over, the first picking was 
made. On October 12, after most of the berries were ripe and some 
new leaves had developed, a second picking was secured. In Table 
VIII are given the percentages of alkaloids found in each individual 
plant, the summary of seasonal averages being added at the close. 
Tasle VIII.—Alkaloids, at two different stages, in the leaves of belladonna plants grown 
jrom cuttings from selected parents at Arlington, Va. 
| 
Alkaloidal content (per cent). 
Alkaloidal content (per cent). 
Lot and number. | irst | Second | Average | LOtandmumber. | first | Second | Average 
stage, stage, for | stage, stage, for 
Aug. 18. | Oct. 12. season Aug. 18. | Oct. 12. | season. 
Lot 6w1 Lot 6wi—Contd. 
Wh SEO eee aoa S 0. 573 0.491 0. 532 VAS enwancs 0.602 0.613 0. 608 
Lot 6we: Met. SSS. SESE . 871 . 669 .770 
DP seek ois 5 asic = 512 .525 .517 12: Sakae case . 801 . 829 - 815 
Lot 6ws: 134, Ast. . 680 . 420 - 550 
Pee ae es: allieiasn win aisle . 525 . 525 Beis a5sccppe Sect |soceese- ee - 630 - 630 
7 ee eee . 720 - 093 - 659 ieee eee See 55S .510 . 238 . 374 
Se So Ose sess oe Seen . 496 - 496 ATi Sack aoe SIE Se . 537 . 537 
A a ee eee eee - 606 . 620 - 613 Oi FE eS 35. teebhe 26650). -- <2 tee eles one 
Ne See SS ete . 604 . 488 - 546 
Gib eet. S42 . 837 . 749 - 493 Average...... . 687 -611 . 649 
Average. . 693 . 578 . 634 || Lot 6wie 
PSS Pee ee . 624 . 610 - 617 
Lot 6ws SITS LAD SRY. sak .398 - 565 
foe tes ee .573 - 522 . 548 A: ENE eT eee eee -445 . 445 
225 eae .615 . 580 . 597 
Seee Mee ie ee . 585 . 343 . 464 Average.....- 677 - 484 . 581 
NS RIA ot . 607 . 478 - 543 
Ss a0 aera paeeeeee . 589 . 589 || Lot 7w3 
FR Pi Ss a | ae 315 ~315 Yaa Aes ae -477 . 429 - 453 
| (Re ee Soe . 573 . 442 - 508 5 ee See (ieee en . 794 . 794 
Be oe. ASSP. 516 . 372 . 444 
Average...... -477 - 611 . 544 
Average.....- 575 455 - 565 
Lot 7W4 
Lot 6ws ESAS Be Pts 8) eens | . 587 . 567 .577 
Sees aaa enor 795 . 797 . 796 7A EAS PORE E A REE ES. oar 5 . 507 -557 
Dep eR 745 . 749 - 147 Dot ee SO: - 562 . 525 - 544 
LEA: Seee ee - 631 . 666 - 648 
Average... ..- .770 .773 . 772 Gio SSS LEAL E caste see oe ps asooe ton Sees] eee eee 
TotGw Usa eee e Rasos . 436 . 669 . 553 
J . 770 516 . 643 
nSon SDSS oe Average...... 553 - 597 - 575 
Lot 6wio 
a Sar | . 623 .618 .621 |, Lot 7ws: “ 
Se aoa temcetiel . 660 . 691 . 676 1 Aerts Peres 25 oe - 569 - 569 
SE A eee See a eae . 636 . 636 
Average..:-..- . 641 . 654 . 648 GS. PSLRA LE OS . 584 . 784 . 684 
Diesen. Sia a . 837 759 . 798 
Lot 6wu 6550 2 . 734 . 620 - 677 
(ee eer . 774 . 653 .714 hist gen eee . 639 . 700 . 669 
7th tae SOU02/ laaneweeses . 602 +. et . 657 .578 - 668 
Beer eee et. S508 jee a ea. . 598 
nn eeer . 682 BTo2 717 Average.....- 690 664 677 
Bees tos '661| = 1775 “718 ———_—— 
