EFFECTS OF SELECTION ON ALKALOIDS IN BELLADONNA. 15 
TasBLeE VI.—Alkaloids in the leaves of second-generation belladonna plants from cross- 
pollinated parents at two stages of growth at Arlington, Va., and Madison, Wis., in 
1914—Continued. 
SUMMARY OF AVERAGES FOR EAcuH LOT, INCLUDING RESULTS AT TIMMONSVILLE, S. C. 
Alxaloidal content (per cent). 
Arlington, Va. Madison, Wis. Timmonsville, S. C. 
Lot. 
First Second First Second First 
stage, stage, | Average.| stage, stage, | Average. Stans Average. 
Aug. 18. | Sept. 12. Aug. 4. | Sept. 5. 8 
NOt GWaevso sos eine oie 0. 656 O\G00F|-feee see 0. 783 SMM Vcagoooosss ON99 2B oe 
Moti Gwaos sans - eee 2 oe .61 Be 3"| Re  ese 8 S205 et. ee. Cc eee © 
MOU GWanle tere este . 608 ODA Neem aoe ae 729 (eels meee: BAQF See oe 
AVOTAZO. < ain\:0/-.< 625 556 0. 592 771 784 0.778 916 0.916 
ILO Bosegssseeesose | 602 . 584 593 524 532 528 950 950 
WOES eee as As 429 AQT ARR REN. 822 S40 ck Serstrsle i eee EEE Go Se ee 
MOG 2a ts ere = tee sk 483 TA Tile eee yee ci 674 SIE ease wanes Ciba Mecenseetacs Ge 
IANVCTASOL aha. 5 . 456 . 436 - 446 . 748 . 827 . 788 771 ATTA 
IU MQ nay bee eee EE COE . 538 AGice Ie Past ete .673 AOGTA| Ee sesy ace SOO alae sere 
Motis4g se eA: . 645 Siti bei ee Be . 696 ROZLE| Le ees F852 SE. Shee 
WO tie eee . 496 ATA | cee hee . 530 JOGO Ee aerate s Hos) Isaosacasoae 
LOGS ses A ees 479 AD Talamers ae ee 514 HOO SSE ES STs MOON: aes SS eers 
Average.....-.- 539 443 491 603 678 642 755 755 
Mousses tate fal S 496 OOO esas see 524 OVA | A eee MODs | econ ee 
LOE Bas Sao Sack Se SOLA fale ee RE pea ela ele nie ae 476 De Gn essen meee BRYAN Wome ace one 
Wots72 fo. eee 446 ADO Viel Bikes 2 LI 602 W22| e850 3 SBP GOON nr 
Average........ 471 402 437 534 628 581 634 634 
A study of Table VI will reveal the fact that the plants showed 
marked differences in the percentage of alkaloids produced at the 
different stations. Thus, the general season average of all the plants 
at Arlington was 0.512 per cent, at Madison 0.663 per cent, and at 
Timmonsville 0.804 per cent. At the last-mentioned station, how- 
ever, only one picking was made, which may account in some measure 
for the unusually high percentage of alkaloids found. At Arlington 
the first picking assayed considerably higher than the second, while 
at Madison the second picking was richer. It should be remembered, 
however, in this connection, that the season at Madison is somewhat 
later than at Arlington. Previous experiments have shown that the 
plants are richest in alkaloids when the flowering period is over and 
the berries are beginning to ripen. At this’stage the leaves are small 
but very rich in alkaloids. At Arlington the first picking was made 
August 18, when the plants were in the stage just described, and the 
second picking was made later in the season, when the new growth 
had reduced the percentage of alkaloids considerably. At Madison, 
on the other hand, the first picking was made August 4, before the 
