12 BULLETIN 306, U. S. DEPARTMENT OF AGRICULTURE. 
When the plants were about 3 inches high they were divided into 
three lots. One lot was transplanted to the gardens at Arlington, 
Va., another lot was shipped to Timmonsville, S. C., and a third lot to 
Madison, Wis. The object was to grow at these widely separated 
locations under different soil and climatic conditions plants which 
were obtained from a definite source. Data would thus be secured 
which would serve to indicate whether the transmissibility of the 
alkaloid-producing quality would be affected by external influences. 
Furthermore, it would throw some light on the question of alkaloid 
production as dependent on sunlight, rainfall, and soil conditions, 
which is a question still unsettled. At Arlington and at Madison two 
pickings were made of all the individuals, while at Timmonsville only 
one collective sample was secured from the plants from each individual 
parent. In Table VI the percentage of alkaloids in the individual 
plants at Arlington and Madison is presented, the summary of averages 
and the results from Timmonsville being added at the close. 
TaBLE VI.—Alkaloids in the leaves of second-generation belladonna plants from cross- 
pollinated parents at two stages of growth at Arlington, Va., and Madison, Wis., in 
191. 
Arlington, Va. Madison, Wis. 
| 
Lot and number. First Second First Second 
stage, stage, Average.| stage, stage, Average. 
Aug. 18. | Sept. 12. Aug. 4. | Sept. 5d. 
Lot 6w7 
WE Oe Se Atel eos Mee eae ats Ss oe 0. 783 0.461 0. 622 0. 962 0. 956 0.959 
Pin Se, 2 :, ESR oak ines Son rae 542 570 . 506 . 107 813 
o's Be So SC rR as et PE De ed le = 488 BASS errno eae 781 781 
1 SS ee Se eee eee 791 666 728 . 653 653 653 
See eee cc 2 a ook 414 508 486 . 791 530 710 
OS Se ie S85 DOB SSE? 5 ae (ee eee 851 851 . 765 707 736 
fae. As SENS A ee ee ee 574 620 597 580 791 685 
ee ee ee 716 646 681 599 861 730 
Be a ee ee 837 835 . 836 . 794 583 688 
TORS oe oes UE oS aoe eee 504 653 . 603 - 478 | 461 470 
TA Oe A eS es oe ee ee 708 522 .615 1.020 727 873 
Doe eee: pees Stee Mee, pe ey key Tae 674 340 .557 1.322 1. 027 1.174 
1B epee oaaes or: Exiow rien Re es ee 695 605 . 650 . 867 813 . 840 
HAAN SES ae eens, See ties SEA ABA 588 669 . 628 956 | 908 . 932 
EM ois Sole Be sie ima Bead a, fa ee 4s 525 525 538 | 949 . 793 
Lot 6w 10 
Fae oi tes pS ae aa ee em yi (2 669 541 . 605 736 557 . 646 
D teed Le eA te) REN eee BRA AN 750 691 57733 G5Sivere ae . 653 
SF ee ee 1k aR FS | ORR die fs ot 639 516 577 685 . 829 Sat 
ASA ER, Meme oho Ra ed ea eee ee SAI Re RSA PR, Ok 806 759 - 183 
Ry vies te Ses Seen eee Ral eS 641 573 . 607 822 1. 068 - 945 
6230258. ee AE ei oe 550 541 - 546 762 . 876 - 819 
a SR tO We OAL vs a Res Soe eee RS 7h kl Re ees = 2 . 748 . 740 . 813 - 776 
Pai Sige iter AS Ae ep giant eee a Raye 8 Do x Sori eee eee .507 . 803 . 733 -7 
Tlie ake Ee a Aes At east Se atmeacgen FONE Seen Romy oo lib Seth ehaieke | ath fen, | ene BG | bee Pe dag dS 1. 062 . 908 -985 
AOS Te LS rea SE ae 327 398 362 . 695 838 . 766 
1 ea ne RR ih tes Colle en Araneae cl a eed Mae gosh esa ES sey weer .905 1.115 1.010 
DD eee. eine uk Sek oe ES AE EAE: eee eee nae . 813 a 
pee a ea ig arate | ee dL . aD 943 - 859 
iV: ERAS Bae ee ee ee eee See TN eT ESTAR Dor) PL Oe eee . 554 605 .579 
TE a Se et, pe OR cers =f aa Aue | pee eo ah A ae ape oe . 806 752 -779 
Lot 6wi: 
DE i ie OIE REE tions ies Bed on tel aM eo ' Ah ere aS . 504 488 .521 
>) at LE Ue ert mee ees es TFS LG ie ee Sees . 557 A573) [2 2S - 573 
5 SE SAL Oe Ae oe ene Be eee ae ett 630 . 636 . 633 . 580 759 . 669 
eae Slee bast SEs AE a, SST 597 . 504 . 547 . 781 749 . 765 
Sy tee St ok et ea a aC Se Oe I ere ee eee 26304 eee . 630 
