8 BULLETIN 306, U. S. DEPARTMENT OF AGRICULTURE. 
TasBLeE 1V.—Alkaloids in the leaves of first-generation belladonna plants from close polli- 
nation and cross-pollination of selected parents at two stages of growth at Arlington, 
Va., during the first season, 1913—-Continued. ; 
SUMMARY OF AVERAGES FOR THE VARIOUS LOTS. 
| 
Average alkaloidal content 
Alkaloidal (esse 
Lot. yield of 
parent : 
First | Second 
stage. | stage. Season 
LiptGw, closepullinutedes: 2 22... Anat es Se -‘High....-- 0.672) 0.701 0. 686 
Doi6w, cross-pollinatedt) 2p e. Pe OF eae ---d0...--- - 586 . 602 . 594 
Tot iw,Cclose.pplinatedee ee en ep eae ee ---d0.------ - 595 . 632 -614 
Dotezw, Cross-pollanated se eee Se os enpe rapt esas eee o~-|- =F do..---- 618 611 615 
Tipi'23, cross-pollimated es eee oe ee ee se eee | Low. ---- ADB oh see . 408 
Lot 46, cross-pollinated.....--- oe ee eS aes 3 SA be) SS eae dox..=2 . 407 | . 448 . 428 
The following year, 1914, these plants were again analyzed. The 
first picking was made on August 1, when the berries were be- 
ginning to ripen. This is the stage at which, as shown by other 
- 
experiments, the greatest percentage of alkaloids is present. The’ 
second picking was made on September 5, when the berries had all 
ripened. Table V shows the percentage of alkaloids in each case, 
the averages being summarized at the close. - 
TaBLeE V.—Alkaloids in the leaves of first-generation belladonna plants from close polli- 
nation and cross-pollination of selected parents at two stages of growth at Arlington, 
Va., during the second season, 1914. 
Alkaloidal content (per cent). ~ Alkaloidal content (per cent). 
Lot and number. First Showa Lot and number. First Sora 
stage, stage, | Average. stage, stage, | Average. 
Aug. 1. | Sept. 5. Aug. 1. | Sept. 5. 
Lot 6w, close polli- Lot 6w, close polli- 
nated: || mnated—Contd. 
Le Es oe a) A eee | 1. 030 0. 783 0. 906 
7 Hite eis Man A Bien d eceraeneete - 902 - 792 - 847 
So scene ceteeee 5 SR oe ie ee 1.030 "| Bios ee cee 
AS ee GE eee hee Sse 1.096 - 908 1.002 
Biers ceteereee i Shirts) meee ; 1.043 | . 765 . 904 
Eat si et tee SOPs Stee see eee | 860 657 - 908 
fe Slacssct SALtL Se 744 | 454 -549 
Sas rs 
w= Ayaan - 906 | (isis ee A 
10. bts SSS SS C SSS EE 
bee || Lot 6w, cross-polli- 
12:: | nated: 
13% Locccck Bee co Se | W158 le. cee 
ry eee eS EA 1. 169 | 687 | 923 
eee 3. soo See eee 969 717 | 843 
16-- RS i cheery se) 5 iste | a ane \ow ec chee 
ives hike pao aot 1.074 . 756 | -915 
kaye 614.5% 2 SE oe - 990 -075 - 782 
19_. Bee - 987 - 530 . 758 
20. . - ee nee eta bape fete eS fey. 
21.. Orta eeeeee - 790 - 069 - 677 
22. - 10:22 J. poe es . 804 - 663 | - 733 
73 eae Ye aoe 1. 062 - 647 854 
24... AD ee Hee = oe 771 . 712 741 
Pee 13: EE: Bea 943 - 626 | 784 
ee 14. 2 oe ee 903 572 737 
27. 15. of 810 -657 733 
Ree 1G: feos | Oe eeaeee Peesoe- see Massssse- 
29. . Ls: ee -929) lnc 2-3 cee 
5 eS ee eee 18: 2 3oe3 Ss 728 .621 674 
