4 BULLETIN 306, U. S. DEPARTMENT OF AGRICULTURE. j 
bloom; the third on June 21, when the first berries were developing; 
and the fourth on September 8, when most of the berries were ripe 
and much new growth had appeared in the form of shoots and suckers. 
The season was a very unfavorable one, and the plants made a rather 
small growth, so that during July and August the leaves were too | 
small and sparse to secure samples. For the purpose of comparison, 
Table IIT gives the alkaloidal content of the various samples and the 
average for the season, the average alkaloidal content of each picking 
being summarized at the close. 
Taste III.—Alkaloids in the leaves of individual first-generation belladonna plants at 
various stages of growth at Arlington, Va., during the second season, 1913. 
Alkaloidal content (per cent). Alkaloidal content (per cent). 
| 
Plant Plant | 
No. First |Second| Third | Fourth) Aver- No. First |Second| Third | Fourth) Aver- 
stage, | stage, | stage, | stage, | age for stage, | stage, | stage, | stage, | age for 
May 5. | May 23.|June 21.| Sept. 8. season. May 5. | May 23.|June 21.| Sept. 8. | season. 
YF [ie eres 023167) 05324 5/822 22 e- 0.320 125 | 0.809} 0.601 | 0.625 | 0.716 0. 688 
342 | 0.516 -473 .312 | 0.856 539 12, . 750 . 608 . 582 574 - 929 
343 GOSs| Se ee eae -352 . 863 . 604 12s - 587 . 434 . 569 . 653 - 561 
344 . 754 - 434 7280 \exeeaee - 492 A 2oiiee: Be eels = 25 Beye . 389 549 - 469 
345 . 622 . 542 356 . 683 s00L ; : 
EWis| serge eS ye oe. . 626 130 - 681 
31 538 . 366 . 430 . 523 - 466 7We . 835 . 530 . 507 - 759 - 663 
32 565 565 630) |552. 22 587 7W3 620 563 431 782 599 
33 511 552 364 790 554 7W4 792 777 588 804 - 740 
34 693 607 350 833 621 WWo"|s43452=3 521 699 998 739 
5 513 477 533 767 573 7We 674 721 567 663 656 
36 ASS Ae 3925) eae 415 7W7 369 765 £60 195 Se 631 
37 412 415 BOS aes 444 (MSA Soe 201 le seceeee 645 468 
Ba [reese SOO 25. =. Ae5|": ee 300 7We - 714 389 544 | 5. oe 412 
Rn esee ce 479 702 782 654 7Wio . 634 496 - 489 764 596 
Cait | Peoeos- 404 - 626 773 601 
133). |Soseetce 255 459 934 549 
Wo {2i24_ 42: -365 s59GfIS. See 481 6wi . 541 543 501 666 561 
IOs ifsesetecnl| sclse sess Be GS a ters see 453 OWanaaeeeee 693 8813] Seer 787 
134 492 359 -318 649 455 6w3 - 850 . 641 787 885 791 
ABE S| Soeevaeee 466 - 559 563 529 6w4 . 866 .516 529 856 692 
IBS | hese aoce Seperate - 470 733 | 601 6Ws . 806 . 640 768 829 761 
1376 |OL see 341 552 766 553 6We . 780 . 135 663 733 728 
19, |bescagase 349 . 664 792 601 6W7 739 -917 | 1.318 956 981 
139 566 452 385 815 555 6ws 780 . 921 937 | 1.020 915 
6Wo 833 - 921 901 919 
12) 854 653 737 494 688 6Wio 908 925 927 876 909 
125 480 516 661 717 594 6wu 956 973 993 924 962 
123 760 672 675 638 686 6Wie . 681 574 670 922 712 
124 605 572 G26i|eeeree ee 619 6Wi3 | 1.143 639 852 754 847 
12; D588. | Be as 675 908 543 
SUMMARY OF ALL INDIVIDUAL PLANTS FROM EACH PARENT. 
Parent plant. Average alkaloidal content of leaves (per cent). 
Stage of growth. 
No Nield off |= 37 [so St pa 24 tho Avera 
alkaloids. | irst | Second | Third | Fourth | season. 
stage. stage. stage. stage. 
————q«~ | oqe_i— ($e qq] e_ m/e 
ae OD SOO E DODO Renee Aaais 5 25> cea Re Lowes. 32 0.625 0. 441 0.326 0. 800 0. 501 
Buc Ss eee dee Sune eae oe Aone oon sss Be dOz--s2se 524 - 470 . 488 739 512 
IGE £29 EOS See iiee ae, es: atte see spe Medium... . 529 - 369 495 . 765 - 531 
LO ease SSS SESS ae iets Sees ee Png he seees . 679 -573 -617 - 662 597 
UPN a ae FS SSE PEE Ewe ee eee ek 2 ee epee, es M0ee 4-12 662 547 583 769 617 
DN noone sates Ss See Se SES ote oS ERE SESS EY GQ... 5. 824 740 824 862 811 
i | fe | 
