4 BULLETIN 517, U. S. DEPAETMEXT OF AGEICULTUEE. 
In this second test, while the concentrated filtrate gave satisfactory 
results, the reaction was not as marked as in the case of the first 
fowl, on which the nonconcentrated fluid was used. 
On June 22 a third series of trials with the filtrate was conducted 
upon 6 fowls that had been inoculated intravenously on May 27 
with a culture of Bacterium fullorum. 
Fowls 77, 78, and 81, with two controls, 86 and 87, received in the 
right wattle 0.1 c. c. of the same concentrated filtrate as used before. 
In each case the injected wattles showed some edema within an hour. 
On June 24 fowls 77 and 87 still showed slight edema, while fowl 
81 showed a considerable swelling. The results of these tests are 
shown in Table II, together with data on agglutination test, autopsy, 
and cultures from ovaries made at a later date. 
Table II. — Concentr.atecl fUtrate tested on foirJs 26 doys after- bioeidation. 
Fowl Xo.— 
Edema 
after 1 
hour, 
June 22. 
Edema 
after 24 
hours, 
June 23. 
Edema 
after 48 
hours, 
June 24. 
Autopsy. 
Culture. 
Agglu- 
tination, 
1 :100. 
{ i .... 
Slight... 
Slight... 
Slight... 
Slight... 
Slight... 
+ 
Slight... 

+ 
+ 

+ 
78 
81 
801 
871 
...do 
...do.... 
...do.... 
-f 

1 Controls. 
At the same time that the foregoing test was made a similar one 
was conducted with the original nonconcentrated filtrate. 
Infected fowls 76, 79. and 82, together with fowls 88 and 89, sup- 
posedly noninfected controls, were injected in the right wattle with 
0.1 c. c. of nonconcentrated filtrate. All showed a discernible edema 
shortly after injection. 
On June 23 a slight edema persisted in fowls 76, 79, 82, and 88 
(control). On June 24 fowl 88, the control, still showed a slight 
edema, fowl 76 showed a slight reaction, while fowls 79 and 82 
showed swelling indicative of a fair positive reaction. The results 
of these tests and other data are given in Table III. 
Table III. — Xonconcentrated filtrate tested on fowls 26 days after' inoeulatwv. 
Fowl Xo.— 
Edema 
after 1 
hour, 
J-one 22. 
Edema 
after 24 
hovirs, 
June 23. 
Edema 
after 48 
hours, 
June 24. 
Autopsy. 
Culture. 
Agr]uti- 
nation, 
1:100. 
76. .... 
Slight. 
Slight. 
Slight. 
Slight. 
Slight. 
SMght. 
Slight. 
Sight. 
Slight. 
Slight. 
Slight. 
-1- 
-f- 
? 
- 
+ 
+ 
+ 
+ 
79 
-1- 
82 
881 . .... 
-f 
+ 
sy 1 
1 Controls. 
