TEST FOE BACTERIUM PULLOEUM i:f^rECTION IX FOWLS, 7 
On September 2, fowls 73 and 77 and control fowl 186 were in- 
jected with 0.1 c. c. As shown in Table V edema was present at 
'J4r hours in fowls 73 and 77 and entirely absent in control fowl 186. 
On September 20 the following birds were injected in the wattle 
with 0.1 c. c. of the same bacterial product as used in the preceding 
test: Fowls 73, 77, 78, 79, 81, 82, and controls 55, 74, 88, 89, 90, 92, 
97, 98, and 99. The results of the test are shown in Table YI. 
Table YI. — Further tests with kiUed boiiiUon cultures. 
Edema 
Edema 
Aggluti- 
nation, 
1 : 100. 
Fowl No.— 
after 24 
hours, 
after 48 
hours, 
Autops}\ 
Culture. 
Sept. 21. 
Sept. 22. 
73 
Slight... 
Slight... 
+ 
+ 
+ 
+ 
+ 
77 
X 
78 
+ 
+ 
do 
+ 
+ 
+ 
+ 
+ 
+ 
79 
+ 
81 
+ 
Slight... 
? 
? 
+ 
+ 
82 
+ 
551 
- 


Not 
tested. 
74 1 
+ 
Slight... 
+ 
+ 

881 
+ 
89 I 
- 
- 


Not 
tested. 
921 
_ 


Do. 
991 
^ 
~ 
~ 
■" 
1 Controls. 
On September 23, the following fowls were killed by bleeding 
and blood was saved for agglutination tests. Autopsy notes follow : 
Fowl 73. A hard tiimorlike mass about an inch in diameler is attached to 
the ovary by fibrous threads and vessels. Other small ova show typical ap- 
pearance of iDullorum infection. Bacterium puJloruia was recovered in pure 
culture from the ovary. 
Fowl 77. Ovary contains one ovum the size of a pea, having the appearance 
of an old puUorum ovum. B. pullorum was obtained in pure culture. 
Fowl 78. One ovum the size of a pea and having characteristic color of those 
with pullorum infection. Also several smaller similar ones and a cyst the size 
of a hickory nut which is filled with a colorless fluid. B. puUoruui was obtained 
in pure culture from the ovum. 
Fowl 79. Ovary contains a number of typical pullorum ova. Pure culture 
of B. pullorum was obtained. 
Fowl 81. Ovary contains large ova apparently normal. There are some 
small brownish ova that may or may not be infected. Liver contains a few 
necrotic spots. Cultures from both liver and ovum gave negtive results. 
Fowl 82. Large ova apparently normal. Some small brownish ova that may 
or may not be infected. Liver contains a few^ whitish spots. Cultures from 
both liver and ova gave negative results. 
On September 24, the following control birds were killed by 
bleeding, and serum was saved for agglutination tests: Fowls 86* 
74, 88, 89, 97, 98, and 99. All were normal, and cultures were nega- 
tive except for fowl 88, which had reacted to the wattle test. This 
