BULLETI]N^ 517^ U. S. DEPARTMENT OF AGEICULTUEE. 
bird was found to possess a typical pullorum ovary, and yielded a 
pure culture of Bactermm pullorum. 
In preparation for another set of trials of the test upon artificially 
infected fowls, on August 26, 1915, the following birds received 
intravenous injections of 1 c. c. of bouillon culture of nine strains 
of B. fvllorum mixed: Fowls 28, 67, 85, 182, 294, 204, and 215. 
On September T the following birds were injected intra abdomi- 
nally with 5 c. c. of a similar mixture of strains: Fowls 16, 18, 
32, 34, 36, 37, 38, 39, 45, 46, 47, 48, 49, 64, 65, 69, 76, 178, 190, and 
197. Fowls 28, 85, and 215 died shortly after the injection. 
The birds were tested Avith killed bacterial culture. The results 
of the two tests, with autopsy findings and results of cultures inocu- 
lated from the ovaries, are given in Table VII. 
Table VII. — Two tests icith nonconcent rated lilled enlture. 
Fowl No.— 
Dec. 
7-8. 
Feb. 
9-10. 
Autopsy Mar. 16. 
Cult- 
ures 
Ig 
4- 
+ 
+ 
+ 
+ 
4- 
+ 
+ 
+ 
+ 
+ 
+ 
+ 
+ 
+ 
+ 
+ 
+ 
+ 
+ 
— 
+ 
+ 
+ 
+ 
+ 
+ 
+ 
+ 
+ 
+ 
+ 
+ 
+ 
+ 
+ 
+ 
_ 
+ 
+ 
Trace. 
Trace. 
+ 
+ 
+ 
+ 
Trace. 
+ 
Trace. 
Trace. 
+ 
+ 
+ 
+ 
+ 
+ 
Trace. 
+ 
Trace. 
Trace. 
Trace. 
Trace. 
Trace. 
Questionable 
Positive 
do 
do 
Questionable 
do 
Positive 
Normal 
18 
4- 
32 ::::::::.. 
-i- 
34 
-f 
36:::;:.: 
37 
38 
39 
_ 
45 
do 
do 
47 
Positive 
4>^ 
49 
f. 
6o 
Normal 
Questionable 
Positive 
+ 
Normal 
Positive 
69 
Questionable 
Positive 
178 
do 
_|_ 
190 
do 
197 . 
do 
"^ 
182 
... do 
204 
Questionable 
Normal 
294 .... 
41 1 
Not killed. . . . 
42 1 
.. .do 
Normal 
27S 1 
Died . 
17'^ 1 ... 
Normal 
80 1 
Not killed. .. 
ISI 1 
do 
101 1 
do 
102 1 
.do . 
103 1 
do 
1 Controls. 
SUMMARY OF THE TESTS WITH ARTIFICIALLY INFECTED BIRDS. 
In the course of the experiments recorded in the foregoing, 32 
birds that had been exposed to infection by injection^ of live cul- 
tures were employed. When tested for the first time 29 of these, or 
90 per cent, revealed edematous swellings rated as either slight or 
positive at 24 hours after injection with the diagnostic agent. When 
read at a 48-hour interval, 23, or 71 per cent, of the same birds gave 
