TEST FOR BACTEEIUM PULLOKUM INFECTIOIST IN FOWLS. 
11 
of every degree of swelling, without impljdng that the slighter 
swellings were significant. 
Table VII. — Comparison, of intradermal and agglutination tests. 
Fowl 
No.- 
Intradermal test. 
Agglu- 
tina- 
tion 
test. 
Fowl 
No.- 
Intradermal test. 
Ar.ilu- 
tina- 
tion 
test. 
93 
76 
77 
Whole wattle swollen, X3; droops. . 
Whole wattle swollen, X3 
H-+ 1 £ 1 1 1 1 1 1 
6f3 
52 
99 
60 
95 
Swelling and drooping of feathered 
skin at edge of wattle 
Trace of swelling at lower edge of 
wattle 
- 
73 
72 
SAVollen, X2 
Swelling possibly due to trauma- 
tism, as wattle is very blue 
Questionable swelling of feathered 
skin at edge of wattle 
96 
Swollen, X 2 
29 
59 
Lower half swollen, X 2 
Lower half swollen, XI 5 - - - 
v + 
81 
Lower half swollen XI 5 
Trace of swelling on posterior half 
of wattle 
100 
97 
Lower half swollen, X l-'5 
The results were particular!}^ discordant in the case of fowl 93, 
which had been placed at the top of the list as showing the best 
intradermal reaction, while it failed to give a reaction to the agglu- 
tination test. In view^ of the discrepancy^. Dr. Rettger obtained the 
bird in question, together with three others, for retest and autopsy. 
The results are shown in Table IX: 
Table IX. — Comparison of retesfs and autopsy findings. 
Fowl 
No.— 
Intradermal test. 
Agglutina- 
tion test. 
Condition of 
ovaries at 
autopsy. 
24 hours. 
48 hours. 
93....... 
72 
29 
60 
Slight. 
Swollen X1.5.... 
- 
Normal. 
Do. 
Normal (small). 
Normal. 
Swollen, X2 
The result of the retest and autopsy of birds 93 and 72 is not 
wholly satisfactory. The repetition of the reaction in both cases is 
significant; but, on the other hand, the results of the agglutination 
test and autopsy leaves the matter inconclusive. As to the remain- 
ing discrepancies in Table VIII, the many other cases noted as surely 
the result of freezing indicate that it is not desirable to apply the 
intradermal test w^here there is a possibility of freezing. 
TRIALS BY OTHER INDIVIDUALS. 
In several instances the test product was sent to interested indi- 
viduals on request. One report on the results was received in which 
1,301 birds were tested and 78 gave a positive reaction. The latter 
w^ere retested by the agglutination method, and 70 gave a positive 
reaction. 
