THE NITROGEN OF PROCESSED FERTILIZERS. 
15 
facture of tho base goods the proteins contained therein have been 
changed. This change is shown to be a deep-seated one, since five of 
the compounds which are known to be final products of protein 
hydrolysis by acids are found. This, however, can not be taken to 
mean that the proteins have been completely hydrolysed by the acid 
treatment since it is possible to have present in the product of partial 
hydrolysis of proteins not only the diamino and monoamino acids, 
but also such intermediate compounds as polypeptids, peptones, 
proteoses, etc. 
In this connection the results obtained by use of the Van Slyke 
method, which are given in Table V, are of particular interest. As 
has been already stated, the base goods were extracted (1) with boil- 
ing water and (2) with boiling acid. In the former case only sHght 
further hydrolysis of the materials in the base goods is to be expected 
since the free acid in the fertilizer is extremely weak, and the boihng 
temperature, 100° C, is that which was reached in the process of 
manufacture. In the case of the second extract complete hydrolysis 
of all the proteins or proteinlike materials is certainly to be expected, 
since in addition to the original hydrolysis the material was boiled 
with strong hydrochloric acid for 24 hours, which treatment in the 
case of most proteins is sufficient for complete hydrolysis. The dif- 
ferences in the results obtained from the analyses of the two extracts 
may, therefore, be expected to throw some light on the question of 
the completeness of hydrolysis of the original proteins by the acid 
processing. 
Table V. — Nitrogen forms as determined by the Van Slyke method. 
Form of nitrogen. 
Results expressed in per 
cent of base goods. 
Results expressed in per 
cent of total N in base 
goods. 
H2O extract. 
HCl extract. 
H2O extract. 
HCl extract. 
Total N 
1.610 
11.372 
1.2C8 
.374 
.031 
.111 
.117 
.081 
.543 
.114 
1.610 
1.435 
1.175 
.382 
.074 
.104 
.070 
.117 
.546 
.133 
Total soluble N . . 
1 85. 24 
114.76 
23. 23 
1.95 
6.89 
7.20 
5.06 
33.75 
7.10 
88.64 
111.36 
Amide N 
23.70 
Humin N 
4.61 
Diamino acid fraction: 
Arginine N . . . 
6.46 
Histidine N 
4.38 
Lysine N 
7.26 
Monoamino acid fraction: 
33.92 
Nonamino N 
8.27 
1 Obtained indirectly. 
First it wall be noticed that total soluble nitrogen in the hydro- 
chloric acid extract is 88.64 per cent of the total N, while that of the 
water extract is 85.24 per cent, showing a difference of 3.4 per cent 
soluble N produced by further hydrolysis of the materials in the 
base goods. Correspondingly there is a decrease of insoluble N. 
