24 
BULLETIN 1105, U. S. DEPARTMENT OF AGRICULTURE. 
The flower clusters which were used for the pollination experiments 
were covered with paper sacks just before the first flowers were 
ready to open. Since the flowers of each cluster opened in succession 
it was necessary to inspect them at frequent intervals, usually every 
other day, and pollinate each as it reached the proper stage of maturity, 
which was considered to be when the terminal pores of the anthers 
were open ready for dehiscence. As a rule, where self-pollination 
was employed each flower was pollinated with its own pollen, but in 
a few instances, where pollen was scarce, that of another flower of 
the same cluster was used. In the tests for cross compatibilities 
pollen from another plant was used. The results are given in Tables 
4, 5, and 6. 
Table 4. — Results of hand pollinations in Solanum chacoense, in 1921 and 1922. 
selfed. 
Number of— 
Plant. 
Number of— 
Plant 
' Flowers 
i pollinated. 
Seed balls. 
Flowers 
pollinated. 
Seed balls. 
No.l 

! No. 24 
9 
2 
3 
4 
3 
o 
4 
5 
2 
3 

No. 2. 

17! 
! 8i o 
i \ 
7 i 
No. 25 

No. 3 
No. 7 
! No. 26 
No. 27 


No. 8 
1 No. 29 

No. 10 
5 
oooo 
No.38 

No. 12 
No. 15 
::::::::: { 
! No. 39 
; No. 43.. 

o 
No. 18 
. No. 50 

No. 19 
No. 21 
5 


1 No. 51 
No. 52 


Crosses Between Different Plants WrrmN the Species. 
Number of— 
Parents. 
Number of— 
Parents. 
SSS j *■"•»■• 
Flowers 
crossed. 
Seed balls. 
No.l x No. 2 
No. 2 X No. 5 
7| 
6 ! 
1 j 
8 I j 
7 ! j 
7 
1 ! 
10 i 10 ! 
4 
5 
No. 13 X No. 29 
No. 14 X No.5 
No. 17 X No. 21 
6 
2 
2 
12 
5 
6 


No. 2 X No. 18... 

No. 3 x No. 1 . . . 
No. 1H X No. 1 

No. 3 X No. 18 
No. 18 X No. 2 
No. 20 X No. 18 

No. 3 X No. 21 . . 
6 
No. 3 X No. 40... 
No. 22 X No. 26 

No. 4 y No. 21 . . . 
No. 29 X No. 1 
4 ! 
No. 5 X No. 18. .. 
No. 29 X No. 2 
5 : 
No. 6 X No. 26 
No. 44a X No. 18 
No. 45 X No.3 
No. 47 X No. 29 
9 
No. 7 X No. 21 
No. 8 X No. 3 
6 
5 
4 
6 
2 
1 


n 



2 
2 2 
No. 8 X No. 18. 
No. 50 X No. 2 
1 
No. 9 X No. 5 
No. 50 X No. IS 
No. 50 X No. 26 
1 
No. 11 X No. 5 . . 
6 | 
No. 13 X No. 11. . 
No. 50 X No. 29 
5 1 
No. 13 X No. 18. - 
3 11 
No. 51 X No. 26 
2 
i 
Every one of the 22 selfed plants listed in Table 4 completely 
failed to produce seed balls. Of the 34 combinations in crosses only 
three were successful, and for these every pistil pollinated produced 
a seed ball with viable seeds. It is, of course, not known how many 
of these plants were of the same clon, and for only the three plants 
which set fruit was it shown that there is a full potency of pistils. 
The pollen of all plants available for study was highly potent (see 
Table 3). It appears, therefore, that there are both self-incompati- 
