44 BULLETIN 918, IT. S. DEPARTMENT OF AGRICULTURE. 
as it is in Egypt in Mar and June (107° and 111° F.) and our bolls 
received some protection from the sun by the screen covering of 
the irrigated bolls and the cheesecloth covering over the nonirri- 
gated bolls. Furthermore, our bolls were probably kept wetter 
during the 30 days when they were wet than Willcocks's were, but 
his were kept wet for a longer time. There was but very little larval 
emergence in any of our bolls. This is shown by the large numbers 
of dead larvae found in situ in the earlier examinations, when they 
were still recognizable, and by the few larvae or pupal skins found in 
the surrounding soil. There was more larval emergence from the 
bolls in the irrigated plots where 12 pupal skins were found in the 
soil than in the nonirrigated where only 2 or 3 were found. About 
half of the larvae recovered in the bolls were in the lint, but no count 
was kept of this point and it is not known whether there were more 
than are usually found in lint in the bolls in the fields during the 
fall or not. In the case of the double seeds in the irrigated plot the 
following note was made: ^Earthen cells were noticed in a few 
instances, apparently some of the larva? left the seed." In the double 
seed on the nonirrigated plot 4 pupal skins were found in the soil. 
On March 11, 1919, 100 bolls which were picked on December 7, 
1918, and stored in the laboratory till this date were buried 1 to 2 inches 
deep in a box of soil and wet thoroughly. The box was sprinkled 
often enough to keep it thoroughly wet for 30 days. On April 11 
the contents were carefully examined and 35 live larvae and 90 dead 
larva? were found in the bolls and only 4 larvae and 2 pupae in the 
soil. Willcocks (7) found a very sudden increase in the number of 
larvae emerging to the surface in a box of bolls buried in dry soil and 
watered in the spring. 
Unfortunately a sufficiently large sample of bolls for our check 
was not examined at the begimiing of the experiment to determine 
the mortality until this date, and the check sample examined at the 
end of the experiment contained more live larvae than at the begin- 
ning. The indications were, however, that the treatment had killed 
a large number of the larvae. The bolls were totally rotted and 
larvae which were dead at the beginning of the experiment were 
unrecognizable at the finish. 
MORTALITY OF HIBERNATING LARVAE IN STORED SEED. 
The number of larvae found in the ginned seed is very small com- 
pared to the number found in the bolls when the cotton is picked. 
In the Laguna the seed cotton is passed through cleaners or beaters 
before it goes to the gins to remove the trash and dirt. This causes 
most of the larvae to leave the lint and many are undoubtedly driven 
from the seed also during the process. Larvae are thrown out by 
the thousands with the trash that comes from the later pickings, and 
in some cases the pile of trash and the sides of surrounding buildings 
