34 
BULLETIN 1429, U. S. DEPARTMENT OF AGRICULTURE 
denced by its extremely localized distribution in Japan and Chosen, 
would tend to limit greatly its possible usefulness in America. 
BREEDING METHODS AND SHIPMENTS 
Methods of breeding were largely those used by other workers with 
this group of parasites. Single females were placed in 3-ounce tin 
salve boxes which had been filled with soil and into which two or three 
Popillia grubs had been placed (fig. 25) . As food for the wasp a drop 
of honey or sugar water was placed on a leaf on the surface of the soil. 
These tins were then set aside for a day and at the end of that time 
Fig. 24.— Tiphia popilliavora, female 
examined, the grubs bearing eggs being removed and fresh ones sup- 
plied. Those bearing eggs were transferred to cross-section trays 
which provided a single compartment for each grub, and in which had 
been placed soil with a small piece of sod to provide food for the grub 
during the period elapsing before its death from the attack of the 
parasite larva. The method of handling grubs parasitized by 
Tiphia differs from that used with Scolia and Campsomeris, because 
the grubs are not permanently paralyzed as they are in the latter two 
genera. During the earlier periods of this work, the soil in the trays 
was kept fairly moist; but it was found that this was responsible for a 
considerable mortality among the grubs by fungus attack, and con- 
sequently in the later work no moisture whatever was applied, and 
improved results were obtained. 
