THE PARASITES OF POPILLIA JAPONIC A 19 
approaching it from behind. During this performance they always 
stand high on their legs and curve the abdomen under and forward. 
However, careful examinations of beetles after these actions have 
failed to reveal larvae upon or within them. Living first-stage larvae 
of this fly in one instance were deposited on the glass of the breeding 
tube, but when transferred to beetles they were soon lost. The first- 
stage larvae (fig. 16, A) are very delicate and can not stand much 
exposure to dry air. Examination of the female flies fails to show 
any piercing organ by which the larvae could be introduced into the 
host. It is possible that the larvae may enter either through the 
genital or anal opening, or if they gain access to the softer body wall 
under the elytra they may penetrate there. The mouth parts of 
these first-instar larvae (fig. 16, B) are not specialized in any way as 
in Centeter cinerea. 
The puparia of this parasite (fig. 14, C) are formed within the 
beetle in the same position as those of Centeter, and like it are buried 
in the ground within the air cavity formed by the body of the dead 
beetle. The adults when emerging push away the dorsal portions 
of the last abdominal segments. The period of pupation ranges 
from 11 to 13 days, with an average of 12 for the first part of July at 
Yokohama. 
The lack of definite data concerning the time of parasitism of the 
host makes it impossible to determine exactly the length of the entire 
life cycle. However, adults have been obtained as late as 20 days 
after the collection of parasitized beetles. 
This tachinid has two, and possibly three, generations a season 
under Yokohama conditions. Adults have been found as early as 
May 24 in Chosen and as late as September 3 at Koiwai and Sep- 
tember 13 at Yokohama. 
At Yokohama this fly has also been reared from adults of Anomala 
rufocuprea Motsch. and (Anomala) Phyllopertha orientalis Waterh., 
and in Chosen from Popillia atrocoerulea Bates and P. mutatis Newm. 
CHAEACTERS FOR DETERMINING THE IMMATURE STAGES OF OCHROMEIGENIA 
ORMIOIDES 
First-stage larva (fig. 16, A). — Length of newly hatched larva approximately 
0.5 mm.; color white, pellucid. Sensory papillae prominent. Mouth hooks 
present; pharyngeal plates as in B. Visible tracheal system in two parallel 
tubes; caudal spiracles stalked. 
Third-stage larva (fig. 16, C). — Length approximately 9 mm.; color white. 
Mouth parts as in D and E. Posterior spiracles raised on a disk which bears 
from three to four hooklike spines; spiracular openings usually four in number. 
Puparium (fig. 14, C). — Length 6 mm.; color dark brown, shining. Posterior 
half swollen. Caudal spiracles elevated and spined as in the larva. 
EUTRIXOPSIS JAVANA Townsend 
Eutrixopsis javana (fig. 17) was first reared at the Japanese 
beetle laboratory in June, 1923, from a shipment of Centeter cinerea 
material forwarded from Sapporo, Japan, in 1922, and comprised 210 
Eutrixopsis out of a total emergence of 6,734 tachinids, or 3.1 per 
cent of the total. However, since all the beetles collected for this 
shipment were those bearing C. cinerea only, it is not possible from 
this collection to make any statement as to the status of Eutrixopsis 
in Japan, although it is undoubtedly secondary in importance to the 
species previously mentioned. Whether it deposits eggs which are 
