26 BULLETIN 395, U. S. DEPARTMENT OF AGRICULTURE. 
SUMMARIZED REPORT OF ELBERTA rXOCULATIOXS. 
A brief summarized report of the Elbert a fruit iuo dilations is given 
below (PL V, figs. 1 to 5). 
Sources of inoculation. — Cladosporium carpophilum from cultures and directly from 
abundantly sporulating lesions on peach twigs, and undetermined fungus A, tinde- 
termined fungus B, and Dematium sp. from cultures. Only young, vigorously sporu- 
lating Lima-bean agar slant cultures of single-spore strains isolated from peach twigs 
were used. 
Methods. — Fruits bagged on May 8 were inoculated by the methods previously 
described in four series on May 16. May 20. May 27, and June 6. respectively, and the 
inoculated areas kept intermittently moist until maturity. 
Results. — In a few cases the experimental fruits were shed or were lost by accidental 
mechanical injury, while in a few of the earlier direct inoculations the spores used 
failed to germinate. These inoculations are not included in this summaiy. 
On 16 areas inoculated with undetermined fungus A, 16 with undetermined fungus 
B, and 16 with Dematium sp. no infection resulted. Consequently, these inoculations 
may be considered as supplementary controls. 
On the 100 control areas of the four series, including as controls the 4S inoculations 
with the superficial fungi mentioned in the preceding paragraph, not one scab infec- 
tion developed. Upon the entire protected surfaces of the 50 fruits in question only 
one lesion appeared, this being in all probability the result of a chance infection 
subsequent to the time of inoculation. Upon these 50 fruits the total number of 
infections was only 21. while 35 matured without showing any evidence of the disease. 
On the IS areas inoculated with Cladosporium carpophilum from pure cultures. 707 
typical infections appeared, an average of 39 lesions on each inoculated area. Only 
one of the IS inoculations failed to result in definite and satisfactory infection, this 
being undoubtedly due to some local imperfection in the conditions of the experiment. 
In only two cases did the disease develop on uninoculated areas, sparse infection 
occurring in both instances near the peduncles, well away from the inoculated surfaces. 
On the six areas directly inoculated with Cladosporium carpophilum from twig 
lesions. 63 typical lesions developed, every inoculation producing decisive infection. 
Only one lesion appeared upon the uninoculated areas of the four peaches in question. 
As these inoculations were made in two corroborative series and as four of them were 
situated upon the protected equatorial surfaces of the fruits, they were doubly guarded 
against natural infection and thoroughly justify positive conclusions. 
COXETRMATORT EXPERIMEXTS OF 1914. 
Experiments conducted at Madison. Wis., in 1914 were planned (1) 
to coirfirai the results of the preceding season in a locality where peach 
scab lias not been observed to occur naturally and (2) to determine 
whether infection may occur during the early stages of development 
of the fruit. The report of these experiments is s umm arized as 
f ollows : 
Sources of inoculation. — Abundantly sporulating young Lima-bean agar cultures of 
single-spore strains of Cladosporium carpophilum isolated from scab lesions of (a x - fruit.. 
(6) twig, and (c) leaf of the peach. 
Methods. — On May 29. about a week after the shedding of the calyces, young Carman 
fruits about 8 mm. in diameter were inoculated, the methods of the Elberta inocula- 
tions of the preceding season being used throughout. The areas of inoculation and 
control were kept intermittently moist until June 23. when the bags and the moisture 
apparatus were removed and the fruits left free. Four fruits were inoculated from 
