2 BULLETIN 893, U. S. DEPARTMENT OF AGRICULTURE. 
tions upon them were performed by G. L. Garrison. The germination 
tests were made by W. L. Goss, of the Seed-Testing Laboratory of the 
Bureau of Plant Industry, and the fungicidal results were determined 
by J. Monteith, jr., and H. F. Bain, of the Federal Horticultural 
Board, and C. C. Thomas, of the Bureau of Plant Industry. 
EXPERIMENTAL WORK. 
GENERAL PROCEDURE. 
Nearly 800 fumigations, in which 20,000 insects of about 15 different 
species were fumigated, were made. The following were fumigated 
in smali glass cylinders, both ends of which were covered by cloth 
held in place by rubber bands: Ants (Tetramorium caespitum Fab., 
Monomorium minimum Buckley, Monomorium pharaonis L., Lasius 
niger L., var. americanus Emery); bedbugs (Comex lectularius L.); 
bedbug eggs; potato beetles (Leptinotarsa decemlineata Say and 
Epitric cucumeris Harris); flat grain beetles (Laemophlaeus minutus 
Oliv.); sawtooth grain beetles (Silvanus surinamensis L.); grain 
borers (Rhizopertha dominica Fab.); flies (Phormia regina Meig., 
Calliphora vomitoria L., Musca domestica L., Stomoxys calcitrans L., 
Chrysomyva macellaria Fab., and Lucilia sericata Meig.); roaches 
(Blattella germanica L.); roach egg pods; flour weevils (Tribolium 
ferrugineum Fab.); and rice weevils (Calandra oryzae L.). The fol- 
lowing insects were fumigated on their hosts: Aphids (M/yzus persicae 
Sulz.); white fly (Aleyrodes vaporarwrum Westw.); Phyllostachys 
bamboo mite (Tarsonemus sp.); and red spider (Tetranychus bimacu- 
latus Harvey). : 
Checks were kept on all insects, and no results are given for insects 
whose check showed any dead. The observations were begun when 
the insects were removed from the fumigatorium, and continued up 
to the time the percentage of dead became constant for any two 
successive days. The checks and treated insects were kept under 
the same conditions until the final observation was made. All of 
the insects, except ants, aphids, red spiders, and white flies, were 
held in the container in which they were fumigated. The bedbug 
eggs were fumigated and kept in chiffon-covered pill boxes, in which 
the eggs had been deposited. After fumigation the ants were re- 
moved to tumblers containing moist sand. Having been fumigated 
on their hosts, aphids, red spiders, and white flies were transferred 
to glass tumblers containing moist sand and fresh uninfested material. 
Roach egg pods were handled in the same manner as ants. 
The following fungi were treated: Fusarium, Ascochyta, Peni- 
cillium, Colletotrichum, and Sclerotium. In some cases_ these 
fungi were placed on a petri dish in a drop; in others, sterilized seeds 
were inoculated by a spore suspension, and exposed in the same man- 
ner as the insects. 
