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EFFECTS OF PORK-CURING PROCESSES ON TRICHIN, 21 
The meat was fed to 4 rats, all of which escaped infection. Portions 
of meat from the same lot of sausage which was freed from its salt 
content in Chicago before being shipped to Washington were fed to 
4 rats, one of which became slightly infected, only 12 encysted 
trichine being discovered in the entire diaphragm. Portions of 
sausage from this lot were fed to 3 rats at the taboratory of Estab- 
lishment 2A and to several mice at the bureau’s laboratory at Chicago, 
but none of these animals developed an infection. 
In Experiment 173 4 rats fed in Washington on sausage meat 
freed from its salt content in Chicago did not become infected. 
Of 4 rats which were fed portions of sausage forwarded to the Wash- 
ington laboratory 3 escaped infection and 1 developed a moderate 
infection. Portions of sausage of the same lot were fed to experi- 
mental animals at Chicago without producing infection, as follows: 
Three rats at the laboratory of Establishment 2A, 3 mice at the 
laboratory of Establishment 3, and 3 mice at the bureau laboratory. 
This makes a total of 17 animals to which this meat was fed, of which 
only 1 developed an infection. In view of the negative results at 
three different laboratories at Chicago, the one positive result at 
Washington appears rather questionable, particutarly because, ac- 
cording to the records, the intact sausage produced the infection, 
whereas the washed sausage meat yielded negative results. 
The details of feeding tests in the 2 other experiments which 
yielded positive results are as follows: 
In Experiment 120 portions of the intact sausage were fed to 3 rats, 
none of which became infected. The sausage meat deprived of its 
salt content in Chicago was fed to 4 rats at the Washington labora- 
tory. Of these 1 escaped infection, 1 developed a moderate infection, 
~ and 2 developed very shght infections, showing 1 and 4 encysted 
larvee, respectively, in their diaphragms. Three rats to which 
sausage from this test was fed at the establishment laboratory in 
Chicago escaped infection. Several mice fed at the bureau labora- 
tory in Chicago were also negative. 
In Experiment 171, 4 rats fed portions of several sausages shipped 
to Washington escaped infection. Sausage meat freed from its 
salt content at Chicago and shipped to Washington was fed to 4 rats, 
1 of which escaped from the cage and was not afterwards found. Of 
the remaining rats 2 became heavily infected and 1 developed a mild 
infection. Three mice were fed at the laboratory of Establishment 
3, of which 1 became infected and 2 escaped infection. ‘Two addi- 
tional lots fed in Chicago escaped infection. 
Summarizing the results of the feeding experiments with this 
variety of sausage, 4 tests yielded positive results, which in case of 
Experiment 173 is of doubtful validity and for which extraneous 
infection may have been responsible. The results of Experiment 119 
