14 
BTLLETIX 969, TJ. S. DEPARTMENT OF AGRICULTURE. 
The minimum lethal dose for these animals was 1.323 pounds. 
This is probably a rather accurate figure, for Sheep 575 received 
1.058 pounds and Sheep 580 1.235 pounds without fatal results in 
either case. The minimum toxic dose was 0.882 pound. Xo experi- 
ments were made with slightly smaller doses, but as Sheep 568 was a 
mild case this dose must be not far from the minimum of toxicity. 
Leaves. — Table 5 shows the dosage when leaves only were fed. 
Table 5. — Dosage of A. mexicana, leaves only. 
Quantity 
Animal. founds™ Res ^- ***«*• 
animal. 
Quantity 
fed per 100 Result 
pounds of, - n - e -"- t - 
animal. 
Pounds. 
Sheen 582 0.176 Xotsick. i Sheep 603 
Pounds, j 
Sheep 5SS 0.353 Sickness. j Sheep 610 
0.S82 Death. 
Sheep 595 0.529 do. 
As Sheep 603 was not very sick on 0.705 pound, it is probable that 
0.882 pound, which caused the death of Sheep 610, is close to the 
lethal dose. While 0.353 pound was the smallest dose producing 
sickness, it is possible that it is not the miuinium, as there were no 
tests between that and 0.176 pound which was given without effect 
to Sheep 582. 
Ste77is. — Nine sheep were fed on stems from which all leaves had 
been removed, as shown in Table 6. 
Table 6. — Dosage of A. mexicana. steins only. 
Animal. 
Sheep 607. 
Sheep 563. 
Sheep 579. 
Sheep 5S1. 
Sheep 5S4. 
, Quantity 
fed per 100 
pounds of 
animal 
Result. 
Pounds. 
0. 705 
0.882 
1.05S 
1.323 
1.411 
Not sick, 
do. 
do. 
Slight sickness. 
Not sick. 
Animal. 
Sheep 586. 
Sheep 595. 
Sheep 602. 
Sheep 603. 
Quantity 1 
fed per 100 
pounds of | 
animal. | 
Result. 
Pounds. 
1.764 Sheht sickness. 
1.481 ! Not sick. 
2.205 \ Symptoms. 
3.527 Possible symptom; 
The smallest quantity of stems fed which produced symptoms was 
1.323 pounds. As compared with this, Sheep 584 and Sheep 595 
showed no effect from 1.411 pounds and 1.481 pounds, respectively. 
The table shows also that as much as 3.527 pounds was fed to Sheep 
603 with only slight effect. No animals were killed by the feeding of 
stems. It appears then that as little as 1.323 pounds may produce 
slight symptoms. The lethal dose has not been determined, but it 
probably is several times this quantity. 
For comparative purposes the toxic and lethal doses of whole plant, 
leaves, and stems to 100 pounds of animal may be tabulated as 
follows : 
Minimum toxic 
dose. 
Pound*. 
Leaves 0. 353 
Whole top 0. 882 
Stems .... 1. 323 
Minimum lethal 
dose. 
Pounds. 
0.882 
1.3 
More than 3.5 
