16 BULLETIN 666, U. S. DEPARTMENT OF AGRICULTURE. 
As has been noted, the water-soluble matter of the alkali-treated 
cocoas has present a protein which is insoluble in 1 per cent sul- 
phuric acid. This varies from 1.15 to 18.20 per cent of the solid 
matter. The greatest variation, however, is shown in the nonash, 
nonprotein matter, which varies from 39.87 to 57.96 per cent, with 
an average of 45.83 per cent, on the untreated cocoas, and from 23.59 
to 48.22 per cent, with an average of 40.64 per cent, on the treated 
cocoas. Kleven samples of treated cocoas contain a smaller per- 
centage of ash- and protein-free extract than the minimum of the 
untreated cocoa. This shows still more plainly what was shown in 
the analysis of the cocoas, namely, that the alkali treatment inhibits 
the solution of this nitrogen-free extract. On the other hand, the 
alkali treatment unquestionably increases, to a slight extent, the 
solubility of the protein. 
ASH RATIOS IN UNTREATED AND TREATED COCOAS. 
The ratio of the water-soluble ash to the water-insoluble ash was 
calculated on both the original cocoa and on the water-soluble matter; 
also, the ratio of the alkalinities of the soluble and insoluble ash was — 
calculated on each of these. These results are listed in Tables 10 
and 11. 
TABLE 10.—Ash ratios on untreated cocoas. 
Alkalinity water- Alkalinity water- 
Water-soluble ash soluble ash Water-soluble ash soluble ash 
Water-insolubleash|} Alkalinity water- Water-insoluble ash) Alkalinity water- 
insoluble ash insoluble ash 
No. NO: ——— SS 
Water- Water- Water- W ater- 
Cocoa. soluble | Cocoa. soluble Cocoa. soluble Cocoa. | soluble 
matter. matter. matter. matter. 
iLO ower 0.55 1. 26 0. 61 OS633| 955 eee eee 0. 45 2. 28 0. 48 0. 72 
23 70 1. 06 49 O15 6 eee ee A9 1. 43 4 75 
25 56 125 47 BOQ" Sitka os fee 38 1. 88 39 75 
32 71 223i 52 TOSS (MoS inaaeee eee 39 1.19 | 46 59 
as sae 405) 1. 81 44 36 | _i— | Kime 
SOR . 60 95 . 46 44 Maximum. 5 fk eo . 61 1. 04 
Ai) oe. 49 5 it) . 42 . 48 Minimum. - . 28 BOO . 34 4380 
41 Ol - 93 . 04 51 } Average.... Sort 1. 24 | +t . 59 
42 - 28 1. 03 . 48 53 —————————| >| 
43 . 62 aY/ 44 AQP AE ek sph.) Dek eed 1.09 1. 82 65 53 
44_ . 62 1. 24 41 Le | aS eee, oe es 3. 46 . 93 1. 66 
45 .45 1. 02 .44 AQF 2O R= eee 16 Mk 1. 46 573 - 93 
AT | 3 1.08 39 {60 SO Re ea eee 1e23 2. 23 Ee Lidl 1. 54 
Se 50 . 80 -43 BOI veo eee ne 3. 16 4.70 1.38 2. 43 
51 45 68 39 AGP ALA oes A oe ee 1. 26 2.45 | . 74 122 
54 39 | 1.18 .36 AG TEI dei ates 2 ee . 24 320) 19 1. 21 
Except on those samples which were treated with magnesium or 
ammonium carbonate, the soluble ash is invariably greater than the 
insoluble ash on the treated cocoas, the lowest figure (with the 
exceptions noted) being 0.81 and the average figure 1.88. With the 
exception of samples 1, 13, 29, and 30, which have been excluded 
from the general averages throughout on the ground of having been 
contaminated with more or less alkali-treated cocoa, the correspond- 
