BACTERIA IN COMMERCIAL BOTTLED WATERS. 5 
Of 57 foreign springs (see Table V) — 
29 (51 per cent) contained no B. coli in 10 cc quantities. 
28 (49 per cent) contained B. coli in 10 cc quantities. 
25 (45 per cent) contained B. coli in 5 cc quantities. 
21 (37 per cent) contained B. coli in 1 cc quantities. 1 
16 (28 per cent) contained B. coli in 0.1 cc quantities. 
8 (14 per cent) contained B. coli in 0.01 cc quantities. 
2 (3 per cent) contained B. coli in 0.001 cc quantities. 2 
Forty (70 per cent) gave counts of less than 100 on one or more 
bottles after incubation for two days at 37° C. 
Twenty-five (44 per cent) gave average counts of less than 100 
per cc at 37° C. 
The highest count shown at 37° C. was 37,000 per cc. This sample 
gave an average count of 16,000 per cc, and B. coli were found in one- 
third of the bottles examined in 5 cc quantities. 
Two imported waters bearing on their labels the words "bacterio- 
logically pure" gave the following results: 
Sample No. 1; six bottles examined — 
Lowest number of organisms per cc developing on gelatin at 20° C 700 
Average number of organisms per cc developing on gelatin at 20° C 2, 450 
Lowest number or organisms per cc developing on agar at 37° C 300 
Average number of organisms per cc developing on agar at 37° C 1, 250 
4 bottles contained B. coli in 10 cc quantities. 
4 bottles contained B. coli in 5 cc quantities. 
4 bottles contained B. coli in 1 cc quantities. 
2 bottles contained B. coli in 0.1 cc quantities. 
Sample No. 2; seven bottles examined — 
Lowest number of organisms per cc developing on gelatin at 20° C 120 
Average number of organisms per cc developing on gelatin at 20° C 9, 410 
Lowest number of organisms per cc developing on agar at 37° C 40 
Average number of organisms per cc developing on agar at 37° C 482 
6 bottles contained B. coli in 10 cc quantities. 
5 bottles contained B. coli in 5 cc. quantities. 
5 bottles contained B. coli in 1 cc quantities. 
5 bottles contained B. coli in 0.1 cc quantities. 
3 bottles contained B. coli in 0.01 cc quantities. 
Among the organisms which have been isolated from the above 
samples are: B. coli, B. cloacae, B. mycoides, B. paratyphosus B, B, 
aerogenes, B. aurantiacus, M. citreus, B. maritimum, B. ovale, B. pro- 
digiosus, B. fluoresceins liquefaciens, B. fluoresceins non-liquefaciens, 
B. subtilis, and long-chain streptococci. 
Molds of the genera Triehoderma, Penicillium, Cladosporium, 
Citromyces, Fusarium, Actinomyces, and Sporotrichum were identi- 
1 Any potable water supply containing B. coli in 1 cc quantities is considered suspicious by health 
departments and is at once investigated. 
2 Water containing B. coli in 0.001 cc quantities is too suggestive of dilute sewage to be accepted by 
anyone. 
