BACTERIA IN COMMERCIAL BOTTLED WATERS. 7 
The results clearly show that bottled waters can be made to con- 
form to the requirements of the United States Public Health Service 
for drinking water furnished upon trains; that is, that not more than 
one 10 cc sample out of five should show the presence of B. coli. 
TABULATED DATA. 
Table I. — Results of the bacteriological examination of water collected from spring No. 1. 
Colonies of organisms per cc de- 
veloping after — 
Smallest quantity 
were found- 
m. which 
Description of sample. 
2 days' in- 
cubation 
on nutrient 
agar at 
37° C 
4 days' incubation on 
nutrient gelatin at 
20° C 
B. coli. 
Total 
count. 
Liquefi- 
ers. 
At time 
of collec- 
tion. 
2 days 
after col- 
lection. 
Molds. 
"Clean " bottle rinsed with 100 cc ster- 
1,000,000 
900,000 
700,000 
1,000,000 
4,800 
790,000 
840,000 
3.000 
4^500 
410,000 
1,400,000 
540,000 
1,100,000 
1,400,000 
700 
400,000 
1,000,000 
48,000 
38,000 
900,000 
17,000 
800 
120,000 
59,000 
18 
18,000 
90,000 
1,000 
1,000 
10,000 
cc. 
0.1 
.1 
.01 
.01 
1.0 
.1 
.1 
C 1 ) 
C 1 ) 
.01 
.01 
.001 
2.001 
cc. 
0.01 
1.0 
.001 
.001 
5.0 
.01 
.1 
0) 
C 1 ) 
.001 
cc. 
Do 
"Dirty" bottle rinsed with 100 cc ster- 
Do 
16 caps rinsed with 70 cc sterile water . . 
Water used for washing and rinsing 
Do 
001 
Do 
.001 
Water "from creek 100 feet from bot- 
Water from swimming pool, after use 
Water from swimming pool, after use 
12 bottles collected after inspection; 
126,000 
152,400 
5,150 
.001 
i No B. coli were present in 10 cc quantities. 
2 This determination was made at the time the sample was received at the laboratory. 
Table II. — Results of the bacteriological examination of water collected from spring No. 2. 
Description of sample. 
"Clean" bottle rinsed with 100 cc ster- 
ile water 
Do 
12 caps rinsed with 100 cc sterile water. . 
Water from bottling spring 
Do 
Water from creek 5 feet from bottling 
spring 
Do 
10 bottles collected after inspection; 
average results 
Colonies of organisms per cc de- 
veloping after— 
2 days' in- 
cubation 
on nutrient 
agar at 
37° C. 
280,000 
300,000 
870 
137,000 
117,000 
310,000 
297, 000 
2,220 
4 days' incubation on 
nutrient gelatin at 
20° C. , 
Total 
count. 
800, 000 
500,000 
1,100 
110,000 
85,000 
( x ) 
C 1 ) 
2,262 
Liquefi- 
ers. 
Smallest quantity in which 
were found— 
B. coli. 
At time 
of collec- 
tion. 
33,000 
100 
2,000 
1,100 
cc. 
0.1 
1.0 
5.0 
1.0 
1.0 
.001 
.001 
2 days 
after col- 
lection. 
cc. 
1.0 
1.0 
5.0 
.1 
.1 
.0001 
.0001 
Molds. 
0.001 
Liquefied. 
This determination was made at the time the sample was received at the laboratory. 
