LEAF-SPOT OF ALFALFA AND RED CLOVER. 
19 
as this. The red-brown color of the Pseudopeziza stroma produced 
by this medium on these white blocks (PL I, B) could be recognized 
at an earlier date than the pale-colored growth which developed 
when other media were tried. 
Spores of Pseudopeziza trifolii were tested in the same way with 
those of P. medicaginis, though not as extensively. The results are 
given in Table VI. 
Table VI. — Viability test of ascospores of Pseu.dopeziza mediaaginis and Pseu- 
dopeziza trifolii viable tvhen dried on plaster of Paris blocks in the laboratory 
and out of doors. 
Place and date of ending 
desiccation. 
Time 
(days). 
Results. 
Place and date of ending 
desiccation. 
Time 
(days). 
Results. 
PSEUDOPEZIZA MEDICAGINIS. 
In laboratory: 
Feb. 1,1915 
Jan 23 1915.. 
32 
36 
37 
38 
39 
48 
49 
63 
66 
72 
76 
76 
79 
85 
93 
94 
100 
104 
210 
357 
27 
34 
52 
77 
78 
106 
+ 
+ 
+ 
+ 
+ 
+ 
+ 
+ 

+ 
+ 
+ 
+ 




+ 


+ 
+ 

+ 
+ 
+ 
PSEUDOPEZIZA MEDICAGINIS— 
continued. 
Out of doors— Continued. 
May 20, 1916 
120 
108 
159 
212 
303 
329 
36 
63 
64 
112 
113 
120 
258 
256 
28 
25 
104 
107 
265 
265 
+ 
Dec 3 1915... 
Feb. 15, 1916 
+ 
Feb 1 1915 . 
Apr. 7, 1916 
+ 
Dec 9' 1916 
May 20, 1915 

Dec 16, 1915 
Sept. 1, 1916 
+ 
Feb 20 1915 
Do 

Mar 26, 1915 
PSEUDOPEZIZA TRIFOLII. 
In laboratory: 
Jan 17, 1916 
Jan 5 1916 .. 
Jan 12, 1916 
Jan 17, 1916 
Jan 23 1915 
+ 
Jan' 22, 1915 . . 
Mar. 7, 1916 
+ 
Jan 1, 1915 
Feb. 15, 1915 
+ 
Aug. 26, 1915... 
Apr 6, 1916 

Do 
May 15, 1916 
+ 
Apr. 11, 1915 
Apr. 20, 1916 

Feb 12, 1916 
Sept. 1, 1916 

Sept. 1, 1916 
Do 

Jan. 15, 1916 
Out of doors: 
Jan. 17, 1916 
_1_ 
Dec 9, 1915 
Do 
+ 
Dec 16, 1915 
May 4, 1916 
+ 
Dec 22, 1915 
Apr. 7, 1916 

Jan 17, 1916 
Sept. 2, 1916 1 

Jan 13, 1916 
Sept. 1, 1916 

Feb 15, 1916 
From these results it appears that drying alone can not be de- 
pended upon to kill all the spores of either Pseudopeziza in less than 
one year. Severe freezing during drying had no apparent effect. 
If conditions for survival are as favorable on the seed as on plaster of 
Paris blocks, the spores should be able to live from one season to the 
next on the seed. But unless conditions on the seed are more favor- 
able than on the block, they should not be able to survive and 
germinate during a second year. 
PATHOGENICITY OF THE FUNGI. 
METHOD OF MAKING INOCULATIONS. 
In all inoculations that have been made, ascospores alone have been 
used as the inoculum. The conidiumlike structures which have 
been described are produced almost wholly in the substrate, and 
since only rarely will one of them separate from the mycelium, no 
