62 BULLETIN 846, U. S. DEPARTMENT OF AGRICULTURE. 
these limits the maximum amount of ammonia nitrogen, as indicated 
by the line, varies from 2.45 to 3.16 milligrams per 100 grams. The 
dots between 22 and 24 per cent of ether extract indicate yolk, and 
in these samples the maximum ammonia nitrogen varies from 5 to 
5.4 milligrams per 100 grams. The encircled dct at the intersec- 
tion of 26.65 per cent of ether extract and 2.7 milligrams of ammonia 
nitrogen represents yolk from the freshest eggs obtainable. The 
corresponding dot for whole egg is at the intersection of 10 per cent 
of ether extract and 1.5 milligrams of ammonia nitrogen. The dis- 
tance below the line at which these dots are located is noteworthy. 
The dots in the neighborhood of 18 and 19 per cent of ether extract 
represent sugared yolk, and the cross at the intersection of 5.7 per 
cent ether extract and 2.6 milligrams of ammonia nitrogen represents 
drip. 3 
As almost all of these results were obtained from August eggs 
when eggs are in the poorest condition, there can be no reason to 
believe that the ammonia nitrogen figures for edible material will be 
appreciably above the line at any season of the year. The line is 
applicable for white, whole egg, yolk, or yolky mixtures made from 
fresh or storage eggs. 
To carry such a plot continually around in order to ascertain 
whether the result for ammonia nitrogen of any sample would fall 
above or below the line would be inconvenient. Moreover, some 
form of expression which can be used in forming a composite picture 
of all of the different analytical results is very desirable. The proper 
mathematical expression for the line is evidently what is needed. 
The expression for such a line is 
Eee ear RR 
ie es yoy — — X’) or he Rh’ = —Q— Q7—g7 @— Q’) 
Widely separated values for &’, Q’, R’’, and Q’’ were selected from 
the plot as follows: 
ih = 0.5 Q’ =0.0 RR’ =5.0 OP 220 
These values establish the position of the line, whether analytical 
results or not. Then’ 
0.5—5.0 
R— 0.5=9 9990 SEM ig 0.0) 
and the vertical distance of any point from this line, in terms of 
milligrams of ammonia nitrogen per 100 grams of sample, will be 
0.5—5.0 
oe k= Sola 0.0) | 
Solving this equation, it becomes 
DR +0205 @0%5 
