64 BULLETIN 86, U. S. DEPARTMENT OF AGRICULTURE. 
well as for whole egg or yolk, prepared from either fresh eggs or 
storage eggs. 
Such a plot of the results in Tables 1 to 23 is given in figure 2, the 
dots and crosses having the same significance as in the ammonia 
nitrogen plot (fig. 1). A line separating them has been drawn, leaving 
six crosses below the line, and one dot above it. If there were six 
dots above the line and one cross below it, the position of the line 
might with justice be criticised. The fact that there are six crosses 
below the line, however, merely shows that, in those particular 
samples, the decomposition was not of a lipolytic nature, but was of a 
proteolytic or fermentative nature. 3 
The dots and crosses between 9.5 and 13 per cent of ether extract 
represent whole egg. Within those limits the maximum amount of 
acidity of the fat, as indicated by the line, varies from 1.94 to 1.97 
cubic centimeters of N/20 sodium ethylate required per gram. The - 
dots between 22 and 24 per cent of ether extract, representing yolk, 
show a corresponding variation for acidity of the fat from 2.04 to 2.06 
cubic centimeters of the N/20 sodiumethylaterequired per gram. The 
dot at the intersection of 26.65 per cent of ether extract and 1.72 cubic 
centimeters sodium ethylate required stands for yolk from the freshest 
eggs obtainable. The corresponding dot for whole egg lies at the 
intersection of 10 per cent of etherextract and 1.49 cubic centimeters 
required. The distance below the line at which these dots are located 
should be noted. The dots in the neighborhood of 18 and 19 per cent 
of ether extract represent sugared yolk, while the cross at the inter- 
section of 5.7 per cent of ether extract and 1.89 cubic centimeters of 
N/20 sodium ethylate required represents drip. 
Again, widely separated values are selected from the plot: 
Q’ =5.0 S22 190. 67980 SEB 
These may be substituted in the straight-line expression 
ei Zon S”’ ; 
to give 
190 20 
S— 1.90=— 9-50.90 (Q—5.0) 
The vertical distance of any point from this line in terms of cubic 
centimeters N/20 sodium ethylate required per gram of fat will be 
: 1.00.— 2.10 : 
Solving this equation, it becomes 
D’ =S —0.008Q — 1.858 
