EXAMINATION OF FROZEN EGG PRODUCTS. 67 
necessity, only to egg material to which no gross addition of foreign 
material has been made and which can be recognized by the fact 
that in white, yolk, whole egg, or yolky mixture, the total nitrogen 
should amount to 12 to 14 per cent of the solids not fat. 
, W x 
10.0D+10.0D’ —25.0D"" + as 000,000 + 500; 500.000 + Y+Z=0 
or 
10.0(R —0.205Q — 0.5) + 10.0(S—0.008Q — 1.858) —25.0 (U+0.029 T 
Ww x 
— 0.748) + V+ 590,000 + 500,000 + ¥ t2=9 
Q=Per cent ether extract, wet basis. 
R=Milhgrams ammonia nitrogen per 100 grams, wet basis. 
S = Acidity of the fat per gram, as cubic centimeters N/20 
sodium ethylate required. 
T= Per cent egg solids not fat, wet basis. 
U=Per cent reducing sugar, as dextrose, wet basis. 
V=5, if indol or skatol present; 0.0, if absent. 
W=Number of bacteria per gram, wet basis, developing on 
plain agar, at 20° C. in 5 days. 
X =Number of confirmed B. colt per gram, wet basis, produc- | 
ing 10 per cent or over of gas in lactose broth, at 37° 
C. in 2 days. 
Y =1 for each mold clump found per 30 pounds af ego material. 
Z=0.5 for each embryo found per 30 pounds of egg material. 
| An explanation of how the values for V; sao Ee ee 
and Z were determined is necessary. 
The value of 5 for V, if indol or skatol was found present, was 
reached by substituting for V different values in the formula until 
the proper value was found. 
The divisor in Tb. to was decided upon by entering on a chart 
(fig. 4), against the number of bacteria present, a dot if the sample 
was considered edible, a cross if it was considered inedible, and a 
dot with a line through it if it was considered of questionable charac- 
ter. It is understood that dividing Wby any divisor and adding the 
result to the formula gives a figure which places a penalty upon the 
presence of any bacteria. This is as it should be, since theoretically 
there should be no bacteria present. Practically, it is impossible, 
unless the aseptic precautions of the bacteriological laboratory 
are employed, to open eggs without introducing bacteria. Such 
aseptic precautions can not be followed in a commercial egg- 
breaking plant. As the magnitude of the divisor determines the 
magnitude of the penalty to be imposed, it should be fairly 
large. It was decided to make the divisor 5,000,000, so that a 
penalty of unity would be placed upon any sample contammg 
