‘ 
86 BULLETIN 846, U. S. DEPARTMENT OF AGRICULTURE. 
If it is necessary to use a spoon to collect the chips made by a bit, 
it should be sterilized in like manner. Wipe all utensils thoroughly 
clean with a cloth before each sterilization. Thoroughly’ cleansed 
Mason jars, which have been sterilized in the autoclave with the 
rubber rings and covers in place, make convenient containers for 
subsamples. 
The bacteriologist records the general condition of all samples at 
the time of sampling, specifying the temperature, appearance, color, 
and odor. 
SUBSAMPLES FOR CHEMICAL EXAMINATION. 
The two chemical subsamples are taken in the same way as the 
bacteriological subsamples, except that sterile instruments and con- 
‘tainers need not be used, and at least 1 pound of material is col- 
lected for each subsample. One of these should be placed in cold 
storage immediately at approximately 0° F. 
SUBSAMPLES FOR PHYSICAL AND MICROSCOPICAL EXAMINATION. 
These consist of the entire contents of each container, after the 
bacteriological and chemical subsamples have been removed. 
Liquip Eaae. 
Thoroughly mix liquid egg samples with a sterilized utensil, such 
as a long-handled dipper which has been immersed, including the 
handle, in alcohol and flamed, after which subsamples are withdrawn 
for examination. Place the subsamples for bacteriological examina- 
tion in sterilized containers. Keep a record of the general condi- 
tion at the time of sampling, specifying the temperature, appearance, 
color, and odor. 
SHELL Eaes.! 
WHOLE EGG. 
Clean each egg? with brush, soap, and water, and immerse for 10 
minutes in a 1:500 mercuric chlorid solution. Transfer with sterile 
crucible tongs to a small conical graduate or other convenient holder, 
acute pole uppermost. Remove .the mercuric chlorid and dry the 
ege by pouring over it first alcohol and then ether. Scorch the 
acute pole to kill spores that may remain. Immediately remove 
the egg from the graduate, holding it near the blunt pole, turning 
the acute pole down. The hands of the operator should have been 
thoroughly greased with vaseline to avoid contamination by bac- 
teria that might rub off during the handling of the eggs. Hold the 
in the laboratories. 
2 Adaptation of method of Bushnell and Maurer, Kansas Agr. Coll. Bul. 201. 
