18 BULLETIN 983, U. S. DEPARTMENT OE AGRICULTURE. 
which the acid was neutralized before drying show no difference be- 
tween neutralized and unneutralized material. 
LEACHED TREATED SAWDUST. 
Duplicate 100-gram samples of digested sawdust were weighed 
into Jena beakers holding 600 c. c, approximately 300 c. c. of water 
was added, and the beakers were placed on the steam bath. After 
30 minutes the solution was filtered, and the filtrate was collected in 
a 2-liter volumetric flask. More water to the amount of 300 c. c. 
was then added to the sawdust, and the heating was repeated, a 
longer time being allowed for each extraction. The total time of 
extraction was 2 days, two of the extractions extending over night. 
The filtrate was' made up to 2,000 c. c, and the latter was used for 
analysis. 
SUGARS. 
Allihn's method of determining the reducing sugars, by means of 
Fehling's solution, as given in Bureau of Chemistry Bulletin 107 
Revised, was followed. The copper oxide was filtered in an asbestos 
Gooch crucible, washed with hot distilled water, and dissolved with 
7 c. c. of concentrated nitric acid. It was then diluted and filtered 
into a 300 c. c. beaker and electrolyzed after the addition of 5 c. c. of a 
saturated sodium acetate solution. Hollard's stationary electrodes, 
consisting of a gauze cathode and a wire-frame anode, were used with 
a current density of 7.5 amperes at 2.4 volts. After all the copper 
was deposited, the electrodes were washed in water, alcohol, and 
ether, dried and weighed, and the dextrose was calculated from the 
copper numbers in the above-mentioned bulletin. 
TOTAL SOLIDS. 
One hundred cubic centimeters of the extract was evaporated to 
dryness in a tared crystallizing dish (in a tannin oven or on the 
steam bath), then placed in the 105° C. oven one hour, and finally 
cooled in desiccator and weighed. 
VOLATILE ACIDS. 
A 100 c. c. sample of the extract was distilled to heavy frothing 
with 10 c. c. of 85 per cent phosphoric acid. Distilled water to the 
amount of 100 c. c. was then gradually added from a separatory 
funnel, as fast as distilled, until the volume of distillate approxi- 
mated 200 c. c. The distillate was then made up to 250 c. c. A 100 
c. c, sample of this distillate was treated in a covered beaker with 
about 2 grams of mercuric oxide for three hours on the steam bath, 
After removal from the steam bath, 10 or 15 c. c. of phosphoric acid 
was added, and the sample was redistilled. Three titrations were 
then made, as follows: On the original sample, which gave the total 
