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or. eT 
BULLETIN No. 989 qj} 
Contribution from the Bureau of Chemistry, W. G. 
CAMPBELL, Acting Chief, and Insecticide and Fungicide 
Board, ie K. HAYWOOD, Chairman. 
Washington, D. C. Vv October 7, 1921 
PINE-OIL AND PINE-DISTILLATE PRODUCT EMULSIONS: 
METHOD OF PRODUCTION, CHEMICAL LO IE Bh Be 
AND DISINFECTANT ACTION. 
By L. P. Surpren, Bacteriologist, and E. L. Grirrin, Assistant Chemist, 
Insecticide and Fungicide Laboratory, Miscellaneous Division, Bureau of 
Chemistry. 
CONTENTS. 
Page. Page. 
Purpose of investigation_________- 1 | Disinfectant action of emulsions of 
ErLOdIChion Of, pine ole = S52 2 pine-oil and other pine-distillation 
Chemical composition of pine oils_- 7 PLOGUCTS = Bee el ee ei as 11 
Hxamination of known samples___- Sta EI WOmGhU STO See ee ae ee ee tay 
Preparation of pine-oil emulsions_- Os eBiblOsrap hye seer eee ater NMEA, 
PURPOSE OF INVESTIGATION. 
The use of a pine-oil emulsion made from “steam” or “steam- 
and-solvent ” process pine oil was advocated by Stevenson (18), of 
the Hygienic Laboratory of the United States Public Health Service, 
in 1915, for general disinfecting purposes. He stated that such a 
product has a Hygienic Laboratory phenol coefficient of 4 to 6, which 
“remains practically constant for about two months. After that a 
noticeable deterioration occurs. Samples four months old show a 
phenol coefficient of about 3.5.” According to McCoy, Stimson, and 
Hasseltine (13), ‘“‘ Hygienic Laboratory pine-oil disinfectant * * * 
is well adapted to the disinfection of intestinal discharges, but ap- 
pears to be deficient against pyogenic organisms,” and “in the pres- 
ent state of our knowledge the use of this preparation should be con- 
fined to bacillary infections such as typhoid fever, cholera, and ba- 
cillary dysentery.” In October, 1917, Walters (31) pointed out the 
fact that pine oil is very much less active than phenol against the 
Staph yl ococcus aureus. 
1The fetes in Jae eee refer to vie hibleananty. at Ate end of this bulletin. 
6317—21 
