20 
BULLETIN 1460, U. S. DEPARTMENT OP AGRICULTURE 
Table 14. — Influence of fineness of division in grinding 1 of sample on protein- 
test results 
PART A 
{Results are on the basis of the moisture as present in sample] 
99 per 
60 per 
30 per 
15 per 
cent 
cent 
cent 
cent 
Whole 
seed 
Maxi- 
Laboratory 
No. passing 
passing 
passing 
passing 
mum 
36-grits 
36-grits 
36-grits 
36-grits 
difference 
gauze 
gauze 
gauze 
gauze 
Per cent 
Per cent 
Per cent 
Per cent 
Per cent 
Per cent 
11998 
10.60 
10.77 
10.66 
10.52 
10.32 
0.45 
11999 
11.91 
11.91 
13.11. 
12.82 
11.80 
12.88 
12.60 
11.66 
12.88 
12.60 
11.63 
13.05 
12.54 
.28 
12000 
12.94 
.23 
12001 
12.78 
.28 
12002 
12.75 
12.71 
12.60 
12.60 
12.37 
.38 
12003 
I 15.45 
15.28 
15. 16 
15.28 
15.16 
.29 
12004 
; 12.20 
12.14 
12.08 
12.03 
12.08 
.17 
12005 
12.75 
12.77 
12.77 
12.65 
12.77 
.12 
12006 
1 14.53 
14.53 
14.25 
14.14 
14.08 
.45 
12007 
10.22 
10.15 
10.15 
10.15 
10.09 
.13 
12008 
10.60 
10.72 
10.54 
10.54 
10.43 
.29 
12009 
10.94 
11.06 
11. 00 
10.83 
10.66 
.40 
PART B 
[Results are on the basis of 13.5 per cent moisture.] 
11999 
12000 
12001 
12002 
12003 
12001 
12005 
12006 
12007 
12008 
12009 
10.47 
10. 53 
10. 51 
10.52 
10.26 
11.62 
11.65 
11.67 
11.66 
11.59 
12.62 
12.71 
12.58 
12.59 
12.80 
12.45 
12.51 
12.40 
12.43 
12.38 
12.42 
12.41 
12.39 
12.40 
12.23 
14.85 
14.78 
14.72 
14.87 
14. 81 
11.75 
11.80 
11.70 
11.75 
11.77 
12.34 
12.45 
12.46 
12.50 
12.55 
14.25 
14.20 
14.27 
14.28 
14.02 
9.84 
9.77 
9.83 
9.88 
9.85 
10.38 
10.41 
10.37 
10.35 
10.31 
10.81 
10.83 
10.80 
10.73 
10.56 
0.27 
.08 
.22 
.13 
.19 
.15 
.10 
.21 
.26 
.11 
.10 
.27 
Eliminating whole seed, it is surprising to find that such con- 
cordant results as are show T n here can be obtained, regardless of 
the fineness of division of the sample. This is attributed to the 
care taken in mixing the sample after grinding, as preliminary 
trials on mill-run material gave very irregular results. Although 
nothing of significance developed (the data from whole kernel ex- 
cepted) from the standpoint of fineness of division, the experiment 
brings out the point that if ground samples are thoroughly mixed 
after grinding, reliable analyses will result, even though the samples 
have not been ground to a fine state of division. It has been the 
experience and observation of the writers that unless the samples of 
the ground material are thoroughly mixed, incorrect results will 
occur. Moreover, grinding to a fine state of division eliminates 
the necessity for extensive mixing. 
INFLUENCE OF MOISTURE CONTENT OF SAMPLE ON PROTEIN-TEST RESULTS 
When samples are ground in an attrition mill in preparation 
for digesting, changes in moisture are bound to occur. How great 
this error will be depends upon such factors as size of sample 
ground, moistness of sample, how cool the mill remains with con- 
tinued use, etc. 
