FURTHER STUDIES OF THE ROTS OF STRAWBERRY FRUITS. 11 
Botrytis sp. or some other fungus." Continued study (12, p. 9) 
convinced the writers that the most common method of entrance of 
Rhizopus was through wounds. 
Later investigations (8 and 9) led Stevens to modify his earlier 
views, and in a paper published jointly with Peterson he states 
(9, p. 264) that " RMzopus nigricans is the fungus which, at the desti- 
nation of the berry, is most in evidence and the fungus which, in 
reality, does most of the damage to shipped berries. * * * It is 
evident that this fungus does not attack sound berries, but is second- 
ary in its nature, following one or another of the fungi mentioned 
above or gaining entrance through some mechanical wound." So 
far as concerns the importance of Rhizopus and the possibility of its 
entering through wounds, this is in substantial agreement with the 
observations of the writers. 
Until the spring of 1917 no good opportunity was afforded for 
studying the effect on Rhizopus infection of previous infection by 
Botrytis. It seemed entirely probable, however, that Botrytis was 
of no particular advantage in the entrance of Rhizopus, since Rhizopus 
is very abundant in strawberries in which there is no Botrytis 
infection. 
During the epidemic of Botrytis on strawberries of the Russell 
variety in the field at Hammond, La., already described, and in 
connection with the experiments on shipping washed and unwashed 
strawberries, advantage was taken of the opportunity to test the 
shipping quality of berries from a field badly infected with Botrytis 
as compared with that of berries from a field in which little Botrytis 
appeared. A single crate was filled, half with berries from the field 
of Klondikes in which there was little Botrytis and half with berries 
from the field of Russells in which Botrytis was so common. These 
boxes were chosen at random from lots which had been sorted and 
packed in the ordinary commercial manner. Half of each variety 
were dipped and half left as checks. All were then shipped to Chicago 
under refrigeration. The results are presented in Table III, tests 1 
and 2. 
While this observation is, of course, complicated by the fact that 
different varieties were used in the two cases, it at least shows that 
there was no marked increase in the amount of Rhizopus present 
in the berries which came from a field badly infected by Botrytis. 
Further evidence as to the relation between these fungi was ob- 
tained by actual inoculation of infected fruit from the same field. 
Berries naturally infected with Botrytis were inoculated with spores 
and mycelium of Rhizopus from pure culture. Berries which had 
only small areas infected with Botrytis were readily inoculated with 
Rhizopus in the remaining sound tissue. In no case was infection 
