SPOROGENES TEST. 
13 
Table 4. — The sporogenes test with milk produced under clean and under dirty 
conditions. 
CERTIFIED MILK. 
Sample 
No. 
Sporogenes test in sets of 10 tubes, with 20 c. c. of milk. 
Posi- 
tive 
tests. 
Bacterial 
count. 
1 
2 
3 
4 
5 
?::::: 
8 
9 
1 10 
i 11 
; 12 
13 
14 
15 
16 
17 
18 
19 
20 
21 
22 
23 
24 
+ 
+ 
+ 
+ 
+ 
+ 
+ 
+ 
+ 
+ 
+ 
+ 
+ 
+ 
+ 
+ 
+ 
+ 
+ 
- 
- 
_ 
_ 
2 
2 

1 




1 
4 
2 
1 
1 
1 

2 

1 




1 
2 
3,900 
4,400 
4,600 
6,500 
8,200 
8,600 
8,100 
9,900 
10, 300 
10, 500 
10, 500 
11,000 
12, 200 
• 12, 600 
12, 700 
16, 700 
18,000 
20,700 
28, 400 
31,500 
31,800 
36,600 
40, 700 
45, 000 
MILK PRODUCED UNDER DIRTY CONDITIONS; COWS DIRTY; UTENSILS NOT 
STERILIZED. 
1 
2 
3 
4 
5 
6 
si;".;; 
9 
10 
11 
12 
13 
14 
15 
16 
17 
18 
+ 
+ 
+ 
+ 
+ 
+ 
+ 
+ 
+ 
+ 
+ 
+ - 
+ 
+ 
+ 
+ 
+ 
+ 
+ 
+ 
+ 
+ 
+ 
+ 
+ 
+ 
+ 
+ 
+ 
+ 
+ 
+ 
+ 
+ 
+ 
+ 
+ 
+ 
+ 
+ 
+ 
+ 
+ 
+ 
+ 
+ 
+ 
+ 
+ 
+ 
+ 
+ 
+ 
+ 
+ 
+ 
+ 
+ 
+ 
+ 
+ 
+ 
+ 
+ 
+ 
+ 
+ 
+ 
+ 
+ 
+ 
+ 
+ 
+ 
+ 
+ 
X 
+ 
+ 
+ 
+ 
+ 
+ 
+ 
+ 
+ 
+ 
+ 
+ 
+ 
+ 
+ 
+ 
+ 
+ 
+ 
+ 
+ 
+ 
+ 
+ 
+ 
+ 
+ 
+ 
+ 
+ 
+ 
+ 
+ 
9 
7 
6 
2 
9 
5 
9 
3 
4 
10 
4 
3 
10 
7 
6 
7 
10 
6 
33,000 
46, 000 
101, 000 
106, 000 
107, 000 
112, 000 
118, 000 
129, 000 
245, 000 
320, 000 
750, 000 
1,340,000 
1,810,000 
2, 900, 000 
11,600,000 
22, 500, 000 
25, 000. 000 
61,000,000 
An examination of the results obtained from milk produced under 
extremely dirty conditions shows that in general the number of posi- 
tive tubes was considerably higher than in the case of the certified 
milk. It is true that there were some samples which showed only two 
or three positive results. Such samples would be difficult to place by 
this test. The general picture presented by the results, however, is 
such that a marked difference is shown in the results obtained by the 
sporogenes test when milk is examined which has been produced under 
these extreme conditions. It is evident that these results determine 
the limitations of the test. The number of positive reactions in a set 
of 10 tubes using 20 c. c. quantities of milk, therefore, must fall either 
within the range of the results shown in Table 4, which represent the 
examination of good and bad milk, or somewhere between these fig- 
