14 BULLETIN 340, U. S. DEPARTMENT OF AGRICULTURE. 
• 
sick animals manifesting the characteristic edema of the throat 
region. It is desired to make particular mention of the boar, a fine 
pure-bred animal, which was in an almost comatose condition, show- 
ing a profuse bloody diarrhea, and a temperature of 106° F. One of 
the sows was also in a very critical condition. 
On the afternoon of June 21 the affected animals were given injec- 
tions of the immune serum, the boar receiving 100 c. c, the sows 50 
c. c, and the shoats 30 c. c. On the following day a visit was made 
to the farm to immunize the remaining hogs, which as yet had shown 
no symptoms of the disease. A total of 138 were given protective 
doses of the serum, the larger hogs weighing 75 pounds or over receiv- 
ing 10 c. c and the smaller animals 5 c. c. Marked improvement was 
noted in the sick animals that had been treated the day before. 
On June 23 another visit was made to the farm. All of the sick 
animals showed still further improvement. The boar was given 60 
c. c. more of immune serum and the sow that had been the most sick 
was given an additional 30 c. c. 
The result of this work was that every affected animal recovered, 
and up to the present time not a single death from anthrax has been 
reported in those animals that received portective doses of the serum. 
In the early part of July an outbreak of anthrax was reported 
from Queen Anne County. Mel. On July 13 two inspectors from 
the bureau were detailed to make an investigation, with a view to 
using our immune serum and spore vaccine in an effort to control 
the outbreak. The disease had made its first appearance about a 
month previous to this time, when a farmer lost a cow from anthrax. 
A few days later a neighbor on an adjoining farm lost a hog from the 
disease. Following this, the disease made its appearance on five 
other farms in the immediate vicinity, the greater percentage of 
animals stricken dying of the apoplectic form of the malady. Ani- 
mals on some of the farms had been treated with single injections 
of a commercial vaccine before the arrival of our inspectors. Immu- 
nization tests were at once started with the bureau serum and spore 
vaccine, with the following results: 
The animals on six farms where losses had occurred from anthrax 
were vaccinated, the cattle, horses, and mules receiving 10 c. c. each 
of serum and 1 c. c. of spore vaccine, except, however, in cases where 
there was reason to believe an animal might be in the incubative 
stage of the disease, when the vaccine was omitted and the dose of 
serum increased. Sheep and hogs on the infected farms were given 
the serum-alone treatment, receiving from 5 to 10 c. c. each. 
On the day subsequent to vaccination a mule on one of the farms 
showed symptoms of anthrax, there being an elevation of tempera- 
ture and a characteristic swelling on one side of the neck, the side 
