EXPERIMENTS IN VACCINATION AGAINST ANTHRAX. 15 
opposite to where the vaccine had been injected. This animal was 
given an injection of 60 c. c. of serum and made a speedy recovery. 
In all, 399 animals, including horses, mules, cattle, sheep, and hogs 
on farms where the disease had broken out, were treated with the 
bureau serum and vaccine. Previous to this an aggregate of 10 
cattle, 3 mules, and 13 hogs had died of anthrax on these farms. 
On the morning of the day following vaccination a cow on one of the 
farms died of anthrax. Exclusive of the above, no losses from 
anthrax have occurred on any of these farms. 
Approximately 140 animals on several other infected farms were 
vaccinated with a commercial vaccine by a representative of the 
State live stock sanitary board. Within a day or two following 
this vaccination it was reported 3 cows and 1 mule died of anthrax, 
and since then 2 more cows have died of the disease. 
Another opportunity was afforded us to test the serum and vaccine 
in an outbreak of anthrax in Noxubee County, Miss., where a num- 
ber of farms were reported to be infected with the disease. A 
quantity of serum and spore vaccine was furnished, and an inspector 
detailed from the bureau station at Birmingham, Ala., to 1 conduct 
the work. On various farms where the disease had made its appear- 
ance a total of 125 cattle were given the simultaneous treatment. In 
addition 3 animals which showed symptoms of the disease were 
given 30 c. c. of serum alone. No deaths from anthrax occurred 
immediately following or since the vaccination, the affected animals 
having all recovered from the disease. 
USE OF SERUM IN TREATMENT OF ANTHRAX IN MAN. 
Extensive data are available on the effectiveness of anthrax serum 
for the treatment of the disease in man. It is recommended that 
from 30 to 40 c. c. of serum be injected in three or four different 
places. Should no improvement follow in 24 hours an additional 
injection of 20 to 30 c. c. of serum should be administered. 
In most instances the results are very favorable, and this treat- 
ment is acknowledged to be superior to any other mode of treatment 
known for this disease. 
CONCENTRATION OF SERUM. 
Experiments are now being conducted in drying immune serum 
with a view to preparing the same in pellet form. For this purpose 
the serum has been dried in shallow pans in a serum-drying appa- 
ratus. After thorough drying it is scraped from the pans, milled 
into a fine powder, and prepared in a pellet machine into proper- 
sized pellets. The spore vaccine is also being prepared in a similar 
