4 BULLETIN 1222, U. S. DEPARTMENT OF AGRICULTURE. 
time of sealing, so that it seems probable that these figures ai 
somewhat too high throughout. At the second weighing (June 30) 
the larva? were seen to differ greatly in size, ranging from 2.3 milli- 
grams to 14.3 milligrams. The largest in the lot was some distam 
from the margin of the frame near a patch of brood about 4 days 
old. On July 1, larvae 3 days old also displayed great differences in 
size. Three larvae from the end of the frame, close to the brood- 
nest, were larger than the other two and weighed 54.4+ milligrams 
each. The two smaller, from the lower edge of the frame, weighed 
only 12.6 milligrams each. These smaller larvae were still being fed 
larval jelly, while the three larger ones were being fed honey and 
pollen. On July 2, great differences in size were still evident, even 
between larvae in adjacent cells. Two larvae were beginning to be 
capped, one of these weighing 164.1 milligrams. The smallest larva 
in the lot weighed only 77 milligrams. On July 3, all larvae of this 
lot were capped except one, which was partly capped. Capped larvae 
of this and the three succeeding lots were not weighed. Weighings 
of 15 mature larvae from this colony during the same season gave 
a total weight of 2,374.7 milligrams and an average of 158.31 milli- 
grams. 
For comparison in the table these weights are used for larvae 4^ 
to 5 days old for Lots 2 to 4, and for larvae 5J to 6 days old for 
Lot 5. 
Lot 3 (June 29). — About 40 cells containing newly hatched larvae 
were selected from the same frame as Lot 2. At the first weighing 
(June 30) the larvae appear quite uniform in size. At the second 
weighing (July 1) the weights ranged from 3.3 to 15.5 milligrams, 
and at the third weighing from 13.1 to 30.3 milligrams. On 
July 3, some cells of this series were completely sealed, one or two 
were partly sealed, but most of them were still open. Near the 
bottom of the frame there were three cells of this lot in a row ; the 
two end cells were sealed, while the middle one was still open. The 
two sealed larvae weighed 158.5 milligrams each ; the one in the open 
cell weighed 117 milligrams. Another larva from an open cell 
weighed only 90.5 milligrams while a fifth weighed 114.3 milli- 
grams. All the cells in this lot were sealed on July 4. 
Lot If. (July 5). — About 50 cells on the same frame were marked, 
great care being taken to mark only those cells containing larvae of 
minimum size. On July 6, 5 larvae weighed 3.05 milligrams, an 
average of 0.61 milligram. On the opposite side of the frame near 
a patch of brood were much larger larvae belonging to this lot, one 
of the largest of these weighing 2.2 milligrams. On July 7 the larvae 
in marked cells continued to show considerable differences in size. 
The larvae of the group isolated among cells containing eggs re- 
mained smaller than those on the opposite side of the comb, located 
next to older larvae. Most of the larvae at this time covered a circle 
one-half to two-thirds of the diameter of the bottom of the cell. The 
total weight of five larvae taken at random from both sides of the 
comb was 31.75 milligrams, an average of 6.35 milligrams. The in- 
dividual weights, in milligrams, were as follows: 2.85, 3.7, 3.7. 9.55, 
11.95. All larvae were still fed with larval jelly. On July 8, the 
weights of 5 larvae ranged from 17.5 to 55.3 milligrams. The 
larger were uniformily near unsealed brood, the smaller among eggs 
near the middle of the frame. The smaller larvae occupied less than 
