50 
BULLETIN 51, U. S. DEPARTMENT OF AGEICULTURE. 
Table 20. — Eggs with blood rings — Continued. 
SMALL SAMPLES OF EGGS WITH LARGE BLOOD RINGS. 
P c3 
Percentage 
Number of bacteria 
r- 
of ammoni- 
£ 
per gram at — 
gen, Folin 
^- 
Sample 
No. 
Date of 
collec- 
tion. 
c3 ^ 
method. 
o 
a 
Size of 
sample. 
Remarks. 
c/5 
m 
-^-^ S 
c3 
^ 
fl 
3 
o 
d 
%r^ 
IJ 
Xi 
>, 
o 
CO 
S 
ro 
^•^•^ 
^ 
fi 
Ph 
1912. 
4292 
Dl 
May 9 
1,700 
400 

3 eggs 
Broken j-olks. 
4401 
D2 
May 27 
ioo 
100 

0.0014 
0. 0052 
72.97 
12 eggs... - 
Do. 
4402 
D2 
...do 
400 
750 
10 
. 0016 
.0059 
72.71 
...do 
Do. 
4430 
D2 
May 28 
100 
150 

.0019 
.0070 
72.71 
5 eggs 
Do. 1 
4699 
04 
July 9 
7,100,000 
400 

.0018 
. 0065 
72.09 
Seggs 
Do. ■ 
LARGE SAMPLES OF EGGS WITH LARGE BLOOD RINGS. 
4838 
F5 
July 25 
4,000,000 
1,900.000 
100,000 
0. 0022 
0. 0077 
71.41 
30 dozen.. 
Kept in chill room 
2 weeks. 
4844 
F5 
July 26 
4,300,000 
3,100.000 
100 
.0019 
.0068 
72.17 
9 dozen . . . 
48S4 
Do 
July 31 
in 1 , 000 
in 1 . 000 
.0019 
.0063 
70.00 
7i pounds. 
4888 
D5 
Aug. 1 
6,500 
Oin 1.000 
10 
.0019 
.0063 
69.96 
10 pounds. 
41040 
D6 
Aug. 22 
2,000,000 
1,400,000 
10.000 
.0020 
.0071 
71.98 
7 pounds.. 
■i 
The results of the first four samples given in the second section of 
Table 20 showed very few organisms and no B. coli except in one 
sample. The amount of ammoniacal nitrogen was identical with that 
found in contemporaneous samples of seconds, cracked, and dirty 
eggs. A fifth sample, taken in July, consisting of eight large blood 
rings, gave a count of 7,100.000 bacteria per gram on agar plates 
incubated at 20° C. and of 400 on similar plates kept at 37° C. The 
divergence of the two counts is not explained. 
In July and Augiist larger lots of both small and large blood rings 
were studied. These were caused in part by the warmth of the late 
summer months and in part by a short period of incubation under 
brood}' hens. The first observations were made of a case of blood 
rings VN'hich had been held in a chill room at about 32° F. for two 
weeks. The eggs were recandled and 8 J- dozen eggs with broken 3'olks 
or with 3^olks stuck to the shell were discarded. During the process of 
breaking, the small and large blood rings were separated, the basis 
of division being less than 3 centimeters for the small and over 
that for the large blood rings. Many of the eggs of the former type 
had firm Vvdiites and yolks w^ith faded rings. The eggs with the large 
blood rings contained broken yolks. All eggs showing signs of mix- 
ing of white and yolk, often termed " runny eggs," were excluded. 
Each lot was mixed thoroughly by passing a few times through a 
steamed sieve. The resulting mixtures had a good odor. The sam- 
ple of small blood rings contained 3G,500 bacteria per gram; the one 
