12 
Bl'LLETIX 51, r. S. DEPAP.T:ME^'T OF AGKICrLmiE. 
EGGS H-\^1NG SETTLED YOLKS. 
Table 5 shows another type of deterioration, which is further ad- 
vanced and more spec-ific than that meant by the general term 
" stale." The yolk has become more opaque and consequently clearer 
in outline when viewed by the aid of the candle and has fallen to the 
pointed end of the shell (see PI. Ill); where it turns sluggishly when 
rotated. Very frequently when these eggs are opened the yolk is 
seen to have a very thin, weak membrane. During warm weather, 
when incubation goes on almost continuously, though very slowly, 
these eggs with settled yolks frequently show a germinal area about 
one-fourth inch in diameter, having a visible white line through 
their center — the "primitive streak" of the embryologists. (See 
PL II. ! Their odor is generally good and their taste not objection- 
able, except for soft boiling or poaching. 
Taele 
-Individual eons irith settled uoJlcs. 
Yolk. 
Whole 
egg. 
Xnmber of 
gas-producing 
bacteria p^er 
B-p.e : ?£-/ 
Total 
number of bacteria T>er gram on 
plain agar. 
gram in 
lactose bile. 
Description. 
Incu- i IncTi- j lEca- 
bated ' bated ' bared 
at20=C.at37=C.at20=C. 
Incu- Incu- ' 
bated ■ bated White. Yolk. 
at 37= Cat 37" C. 
4004 
1911. 
June 12 
June 13 
...do 
...do 
...do 
1912. 
70 


30 
20 

o ooo oo 

o' 




"VSTateFT wtite 
^13 
4014 
4015 
4016 
4018 
410S9-7 
30 
20 
20 
40 
30 
, 

10 ; 
«; - 
10^ 
... 64,000 





Enlarged hatch spot; 
bloated yolk. 
Euiareed hatch spot. 
Enlargf^ hatch spot; 
bloated yolk. 
41089-8 

iOatlOC 
Verv weak volk which 
41089-12 
...do 
54.000 
broke when egg was 
opened. 
410S9-18 
410S9-19 
...do 
do ... 
'."'.'."'. 
'240,000 
lOatlOO 
Marked shnnkase. 
Marked vhrinka^e; volk 
41(^9-21 
...do 
do 

1 
Oat 100 
at 100 
broke when egg was 
opened. 
Marked sbnnkace. 
Decidedly weak yelk 
which broke when 
egg was opened. 
Marked shrinkage. 
41089-28 
...do 
do...- 

1 
Oat 100 
... at 100 
41089-33 
Do. 
41(K9-3S 
...do 
...do 
Oat 100 
at 100 
Do. 
41089-39 
Marked snrmVaP-p; volt 
41089-41 
...do 
...do 
...do 
...do 

........ 
Oat 100 
Oat 100 
Oat 100 
1 
Oat 100 
OatlOO 
;0atl00 
at 100 
broke when egg was 
opened. 
Markpd «:hr7Titra.(7p 
4108^-42 
Do. 
41089-44 
41089-45 

Marked shrinkage: yolk 
broke when egg was 
opened. 
Do. 
41089-46 L..do 
Do. 
41089-50 ! do 
MarkP<l «;hrintagp 
41089-53 
41089-55 
...do 
Do. 
do 
(OatlOO 
Do. 
41089-57 
...do 
OatlOO 
Do. 
41089-58 
...do 

OatlOO 
Do. 
41(K9-59 
do... 
j 
Oat 100 

Do. 
41089-61 
...do 
...do 
...do 
1 
OatlOO 
OatlOO 
at 100 
Yolk broke when egg 
41089-62 
was opened. 
Marked vhrintsigP 
41089-63 
^ at the dilution given indicates that no organisms were observed on the plate. 
