CHANGES IN FRESH BEEF DURING COLD STORAGE. 79 
period was but half as long; while each increase is less than the corre- 
sponding increase in Experiment No. 3, where the storage period was 
but three-fourths as long, and where the preformed ammonia was 
present in the original material in about the same quantity as in the 
present experiment. 
Each portion of the stored quarter contained less total phosphorus 
than the corresponding portion of the fresh quarter. The signifi- 
cance of these apparent decreases is far from being clear. 
Table 53 shows the distribution of nitrogen and phosphorus ex- 
pressed as percentages of total nitrogen and total phosphorus. 
The data for nitrogen do not demand special discussion. 
Changes in insoluble phosphorus are of the usual irregular nature 
and their significance has not been established. 
The changes in soluble inorganic phosphorus are in the nature of 
distinct increases. As regards the amount of preformed inorganic 
phosphorus that it contained, the fresh material is comparable to that 
used for Experiments Nos. 1 and 3. By comparing the increases in 
the inorganic phosphorus ratio in the same three experiments it is 
found that the increases during the 54-day storage period of the 
present experiment are greater, on the whole, than the corresponding 
increases effected by the shorter periods of storage of Experiments 
Nos. 1 and 3, the only exception being that the change in the round in 
this experiment is somewhat less than the corresponding change in 
Experiment No. 3. On the whole, the results are in conformity with 
those obtained in the autolysis experiment. 
Changes in soluble organic phosphorus are of less significance than 
the corresponding changes in inorganic phosphorus. 
EXPERIMENT NO. 7. 
HISTORY OF CARCASS. 
A " grade " Shorthorn steer 4 years old, rather rough in conforma- 
tion and only fairly well finished, was slaughtered in the usual 
manner. The carcass was allowed to hang 1 hour on the killing 
floor, after which it was run into the fore cooler, where it was held 
16 hours, and then into the main cooler, where it was held 51 hours 
at 30° F. The humidity of the fore cooler was 93 per cent and that 
of the main cooler 92 per cent of saturation. The weight of the 
warm carcass was 814 pounds. After storage in the packing-house 
coolers for a total period of 67 hours, the hind quarters were cut from 
the carcass, carefully wrapped, and transported to the bureau's cold- 
storage rooms, where one hind quarter was immediately prepared 
for analysis while the other was placed in cold-storage room No. 1, 
where it was held in storage. 
