CHANGES IN FRESH BEEF DURING COLD STORAGE. 47 
ment being greater than the maximum increase that occurred in ex- 
periment No. 1. These results are in conformity with those obtained 
in the autolysis experiment. 
Slight apparent decreases, which for the present must be regarded 
as due to possible inequalities in sampling, have taken place in the 
total phosphorus. On account of the effect of the decreases in total 
phosphorus upon the value of the other phosphorous compounds^ 
changes in those constituents will be discussed in connection with 
Table 25. 
Table 25 shows the distribution of nitrogen and phosphorus ex- 
pressed as percentages of total nitrogen and total phosphorus. 
The distribution of the nitrogen compounds does not differ greatly 
from that in case of experiment No. 1. There is an appreciable in- 
crease in the proportion of total nitrogen present as soluble, non- 
coagulable, proteose, and ammoniacal nitrogen, and a decrease in the 
proportion present as coagulable nitrogen. 
Insoluble phosphorus shows appreciable decreases that range from 
5.22 per cent in case of the rump to 8.58 per cent in case of the loin. 
These results appear to be in conformity with the findings obtained 
in the autolysis experiment, but in view of the results obtained in 
other experiments of this series this seeming conformity must be 
regarded as accidental. 
Total soluble phosphorus shows increases corresponding to the 
decreases in insoluble phosphorus. 
Soluble inorganic phosphorus shows appreciable increases, which 
range from 10.37 per cent in the case of the round to 23.47 per cent 
in the case of the loin. On the whole the increases in soluble inor- 
ganic phosphorus are but slightly greater than similar changes in 
this constituent in Experiment No. 1. On account of the much longer 
storage period in Experiment No. 2 a larger increase in inorganic 
phosphorus might have been expected; but in this connection it is 
interesting to note that the material used for this experiment already 
contained a considerably higher percentage of preformed inorganic 
phosphorus than did the material used in the first experiment. It 
would appear as though the larger quantity of inorganic phosphorus 
present in the material used in the second experiment either of itself 
retarded the rate of change of organic phosphorus into inorganic 
forms or was indicative of some retarding agency. 
Interesting light is thrown on this question by the results of the 
autolysis experiment, as shown in Table 11, where under the head- 
ing " Inorganic phosphorus " it may be noted that the increase in 
this constituent takes place most rapidly during the first 7 days 
of the experiment. Thus during the first 7 days the relative in- 
crease amounts to 52.78 per cent, while during the total incubation 
period of 100 days the relative increase amounts to only 65.27 per 
