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02 
though they seem to be more nearly 
in conformity with a regular rule. 
On the whole, the rate of ammonia 
production decreases as the am- 
monia accumulates; yet in view 
of the small amounts in which this 
constituent is present (see Table 
9), it would seem that the am- 
monia is but an index of the re- 
tarding agent and not the retard- 
ing agent itself. 
The production of ammonia is 
clearly due to enzym action, but 
the nature of the specific enzym 
and of the mother substance re- 
mains to be determined. 
Table 11 shows the distribution 
of nitrogen and phosphorus ex- 
pressed in terms of percentages 
of total nitrogen and total phos- 
phorus. 
/Soluble nitrogen constitutes 29.8 
per cent of the total nitrogen in 
the fresh material, 23.28 per cent 
in the material incubated for 7 
days, and 36.45 per cent in the 
sample incubated for 100 days. 
The decrease in the ratio of solu- 
ble nitrogen to total nitrogen in 
the samples incubated for 7, 14, 
and 21 days confirms similar data 
presented in Table 10. 
Coaguldble nitrogen makes up 
17.79 per cent of the total nitrogen 
in the fresh material and only 3.52 
per cent in the sample incubated 
100 days. More than 80 per cent 
of the coagulable nitrogen present 
in the fresh material has been con- 
verted into noncoagulable forms. 
Proteose nitrogen constitutes a 
relatively small proportion of the 
total nitrogen both in the fresh and 
in the incubated material. 
